Theravectys, Paris, France.
Unit of Lymphocyte Cell Biology, Immunology Department, Institut Pasteur, Paris, France.
Eur J Immunol. 2021 Jan;51(1):180-190. doi: 10.1002/eji.202049058. Epub 2020 Dec 23.
Although the COVID-19 pandemic peaked in March/April 2020 in France, the prevalence of infection is barely known. Using high-throughput methods, we assessed herein the serological response against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) of 1847 participants working in three sites of an institution in Paris conurbation. In May-July 2020, 11% (95% confidence interval [CI]: 9.7-12.6) of serums were positive for IgG against the SARS-CoV-2 N and S proteins, and 9.5% (95% CI: 8.2-11.0) were neutralizer in pseudo-typed virus assays. The prevalence of seroconversion was 11.6% (95% CI: 10.2-13.2) when considering positivity in at least one assay. In 5% of RT-qPCR positive individuals, no systemic IgGs were detected. Among immune individuals, 21% had been asymptomatic. Anosmia (loss of smell) and ageusia (loss of taste) occurred in 52% of the IgG-positive individuals and in 3% of the negative ones. In contrast, 30% of the anosmia-ageusia cases were seronegative, suggesting that the true prevalence of infection may have reached 16.6%. In sera obtained 4-8 weeks after the first sampling, anti-N and anti-S IgG titers and neutralization activity in pseudo-virus assay declined by 31%, 17%, and 53%, resulting thus in half-life of 35, 87, and 28 days, respectively. The population studied is representative of active workers in Paris. The short lifespan of the serological systemic responses suggests an underestimation of the true prevalence of infection.
尽管 COVID-19 大流行在 2020 年 3 月/4 月在法国达到高峰，但感染的流行率知之甚少。使用高通量方法，我们评估了来自巴黎都市区三个地点的一个机构的 1847 名参与者对严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的血清学反应。在 2020 年 5 月至 7 月期间，11%（95%置信区间[CI]：9.7-12.6）的血清 IgG 对 SARS-CoV-2 的 N 和 S 蛋白呈阳性，9.5%（95%CI：8.2-11.0）在假型病毒测定中为中和抗体。当考虑至少一种测定呈阳性时，血清转化率为 11.6%（95%CI：10.2-13.2）。在 5%的 RT-qPCR 阳性个体中，未检测到系统性 IgG。在免疫个体中，21%的个体无症状。在 IgG 阳性个体中，52%出现嗅觉丧失（嗅觉丧失）和味觉丧失（味觉丧失），而在阴性个体中，这一比例为 3%。相比之下，30%的嗅觉丧失味觉丧失病例为血清阴性，这表明感染的真实流行率可能已达到 16.6%。在第一次采样后 4-8 周获得的血清中，抗-N 和抗-S IgG 滴度和假病毒测定中的中和活性分别下降了 31%、17%和 53%，半衰期分别为 35、87 和 28 天。所研究的人群代表了巴黎的活跃工作者。血清学系统反应的短寿命表明对真实感染流行率的低估。
