Sharifi-Rad Majid, Pohl Pawel, Epifano Francesco, Álvarez-Suarez José M
Department of Range and Watershed Management, Faculty of Water and Soil, University of Zabol, Zabol 98613-35856, Iran.
Department of Analytical Chemistry and Chemical Metallurgy, Faculty of Chemistry, Wroclaw University of Science and Technology, Wyspianskiego 27, 50-370 Wroclaw, Poland.
Nanomaterials (Basel). 2020 Nov 29;10(12):2383. doi: 10.3390/nano10122383.
Today, the green synthesis of metal nanoparticles is a promising strategy in material science and nanotechnology. In this research, silver nanoparticles (AgNPs) were synthesized through the high-efficient, cost-effective green and facile process, using the Delile. root extract as a bioreduction and capping agent at room temperature. UV-Vis spectroscopy was applied for the investigation of the reaction proceedings. To characterize the greenly synthesized AgNPs, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and transmission electron microscopy (TEM) analyses were utilized. In addition, the total phenolics and flavonoids contents, antioxidant, antibacterial, and anti-inflammatory activities of the greenly synthesized AgNPs and the root extract were evaluated. The results indicated that the AgNPs had spherical morphology and crystalline structure with the average size of 34.2 ± 8.0 nm. The total phenolics and flavonoids contents of the greenly synthesized AgNPs were lower than those for the root extract. The resultant AgNPs exhibited the appropriate antioxidant activity (64%) as compared to that for the root extract (47%). The antibacterial test approved the higher bactericidal activity of the resulting AgNPs on the Gram-positive and Gram-negative bacteria in comparison to the root extract. Considering the anti-inflammatory activity, the greenly synthesized AgNPs showed a stranger effect than the root extract (82% versus 69% at 500 μg/mL). Generally, the AgNPs that were fabricated by using the root extract had appropriate antioxidant, antibacterial, and anti-inflammatory activities and, therefore, can be considered as a promising candidate for various biomedical applications.
如今，金属纳米颗粒的绿色合成是材料科学和纳米技术中一种很有前景的策略。在本研究中，以Delile.根提取物作为生物还原剂和封端剂，在室温下通过高效、经济高效、绿色且简便的方法合成了银纳米颗粒（AgNPs）。采用紫外可见光谱法研究反应过程。为了表征绿色合成的AgNPs，利用了傅里叶变换红外光谱（FTIR）、X射线衍射光谱（XRD）和透射电子显微镜（TEM）分析。此外，还评估了绿色合成的AgNPs和根提取物的总酚和黄酮含量、抗氧化、抗菌和抗炎活性。结果表明，AgNPs具有球形形态和晶体结构，平均尺寸为34.2±8.0nm。绿色合成的AgNPs的总酚和黄酮含量低于根提取物。与根提取物（47%）相比，所得AgNPs表现出适当的抗氧化活性（64%）。抗菌试验证实，与根提取物相比，所得AgNPs对革兰氏阳性菌和革兰氏阴性菌具有更高的杀菌活性。考虑到抗炎活性，绿色合成的AgNPs显示出比根提取物更强的效果（500μg/mL时分别为82%和69%）。总体而言，利用根提取物制备的AgNPs具有适当的抗氧化、抗菌和抗炎活性，因此可被视为各种生物医学应用的有前途的候选物。
