Wüst J, Heizmann W, Hardegger U, Manncke B
Department of Medical Microbiology, University of Zürich, Switzerland.
Eur J Clin Microbiol Infect Dis. 1988 Aug;7(4):511-7. doi: 10.1007/BF01962602.
The results of susceptibility tests performed by the Cobas-Bact system were compared with those of the NCCLS agar diffusion (Kirby-Bauer) and NCCLS agar dilution methods. A total of 998 clinical isolates were tested against 10 to 18 antimicrobial agents. Essential agreement (comprising full agreement and minor discrepancies) varied from 90.5% to 99.2% on comparison of Cobas-Bact with Kirby-Bauer results, depending on the bacterial group (mean for all 998 strains tested 95.7%). These figures ranged from 91% to 99.2% (mean 96.3%) for the Cobas-Bact/MIC comparison and from 95.2% to 99.7% (mean 98.7%) for the Kirby-Bauer/MIC comparison. The best results were found for Enterobacteriaceae and Staphylococcus aureus, whereas for enterococci and coagulase-negative staphylococci there was a lower rate of essential agreement in all three comparisons. In the case of Pseudomonas aeruginosa there was a good rate of essential agreement but many minor discrepancies, resulting in a disappointing rate of full agreement of between 67.5% and 78.9% in the three comparisons. The Cobas-Bact system would appear to provide satisfactory susceptibility test results in most cases, however there are still some major problems in the system which should be resolved.
将Cobas-Bact系统进行的药敏试验结果与美国国家临床实验室标准化委员会（NCCLS）琼脂扩散法（Kirby-Bauer法）及NCCLS琼脂稀释法的结果进行了比较。共对998株临床分离菌进行了10至18种抗菌药物的测试。根据细菌类别，将Cobas-Bact系统的结果与Kirby-Bauer法的结果相比较时，基本一致率（包括完全一致和微小差异）在90.5%至99.2%之间变化（所有998株测试菌株的平均值为95.7%）。在Cobas-Bact系统与最低抑菌浓度（MIC）比较中，这些数字在91%至99.2%之间（平均值为96.3%），而在Kirby-Bauer法与MIC比较中，这些数字在95.2%至99.7%之间（平均值为98.7%）。肠杆菌科细菌和金黄色葡萄球菌的结果最佳，而在所有三项比较中，肠球菌和凝固酶阴性葡萄球菌的基本一致率较低。对于铜绿假单胞菌，基本一致率较高，但存在许多微小差异，导致在三项比较中完全一致率令人失望，在67.5%至78.9%之间。Cobas-Bact系统在大多数情况下似乎能提供令人满意的药敏试验结果，然而该系统仍存在一些主要问题有待解决。
