Ahamed Maqusood, Akhtar Mohd Javed, Khan M A Majeed, Alhadlaq Hisham A, Alshamsan Aws
King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Nanomaterials (Basel). 2020 Nov 22;10(11):2309. doi: 10.3390/nano10112309.
Barium titanate (BaTiO) nanoparticles (BT NPs) have shown exceptional characteristics such as high dielectric constant and suitable ferro-, piezo-, and pyro-electric properties. Thus, BT NPs have shown potential to be applied in various fields including electro-optical devices and biomedicine. However, very limited knowledge is available on the interaction of BT NPs with human cells. This work was planned to study the interaction of BT NPs with human lung carcinoma (A549) cells. Results showed that BT NPs decreased cell viability in a dose- and time-dependent manner. Depletion of mitochondrial membrane potential and induction of caspase-3 and -9 enzyme activity were also observed following BT NP exposure. BT NPs further induced oxidative stress indicated by induction of pro-oxidants (reactive oxygen species and hydrogen peroxide) and reduction of antioxidants (glutathione and several antioxidant enzymes). Moreover, BT NP-induced cytotoxicity and oxidative stress were effectively abrogated by N-acetyl-cysteine (an ROS scavenger), suggesting that BT NP-induced cytotoxicity was mediated through oxidative stress. Intriguingly, the underlying mechanism of cytotoxicity of BT NPs was similar to the mode of action of ZnO NPs. At the end, we found that BT NPs did not affect the non-cancerous human lung fibroblasts (IMR-90). Altogether, BT NPs selectively induced cytotoxicity in A549 cells via oxidative stress. This work warrants further research on selective cytotoxicity mechanisms of BT NPs in different types of cancer cells and their normal counterparts.
钛酸钡（BaTiO）纳米颗粒（BT NPs）具有卓越的特性，如高介电常数以及合适的铁电、压电和热电性能。因此，BT NPs已显示出在包括电光器件和生物医学在内的各个领域的应用潜力。然而，关于BT NPs与人类细胞相互作用的知识非常有限。这项工作旨在研究BT NPs与人类肺癌（A549）细胞的相互作用。结果表明，BT NPs以剂量和时间依赖性方式降低细胞活力。在暴露于BT NPs后，还观察到线粒体膜电位的耗竭以及caspase-3和-9酶活性的诱导。BT NPs进一步诱导氧化应激，表现为促氧化剂（活性氧和过氧化氢）的诱导以及抗氧化剂（谷胱甘肽和几种抗氧化酶）的减少。此外，N-乙酰半胱氨酸（一种活性氧清除剂）有效地消除了BT NPs诱导的细胞毒性和氧化应激，表明BT NPs诱导的细胞毒性是通过氧化应激介导的。有趣的是，BT NPs细胞毒性的潜在机制与ZnO NPs的作用模式相似。最后，我们发现BT NPs不影响非癌性人类肺成纤维细胞（IMR-90）。总之，BT NPs通过氧化应激在A549细胞中选择性诱导细胞毒性。这项工作值得进一步研究BT NPs在不同类型癌细胞及其正常对应细胞中的选择性细胞毒性机制。
