Tuberculosis Department of Hohhot Second Hospital, Inner Mongolia, 010020, China; Hohhot Tuberculosis Prevention and Control Institute, Inner Mongolia, 010020, China.
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing 400715, China.
Infect Genet Evol. 2023 Apr;109:105417. doi: 10.1016/j.meegid.2023.105417. Epub 2023 Feb 16.
Phage therapy is revitalized as an alternative to antibiotics therapy against antimicrobials resistant pathogens. Mycobacteriophages are genetically diverse viruses that can specifically infect Mycobacterium genus including Mycobacterium tuberculosis and Mycobacterium smegmatis. Here, we isolated and annotated the genome of a mycobacteriophage Lang, a temperate mycobacteriophage isolated from the soil of Hohhot, Inner Mongolia, China, by using Mycolicibacterium smegmatis mc 155 as the host. It belongs to the Siphoviridae family of Caudovirales as determined by transmission electron microscopy. The morphological characteristics and certain biological properties of the phage were considered in detail. Phage Lang genomes is 41,487 bp in length with 66.85% GC content and encodes 60 putative open reading frames and belongs to the G1 sub-cluster. Genome annotation indicated that genes for structure proteins, assembly proteins, replications/transcription and lysis of the host are present in function clucters. The genome sequence of phage Lang is more than 95% similar to that of mycobacteriophage Grizzly and Sweets, differing in substitutions, insertions and deletions in Lang. One-step growth curve revealed that Lang has a latent period of 30 min and a outbreak period of 90 min. The short latent period and rapid outbreak mark the unique properties of phage Lang, which can be another potential source for combating M. tuberculosis.
噬菌体疗法作为一种替代抗生素治疗对抗微生物耐药病原体的方法而重新受到关注。分枝杆菌噬菌体是具有遗传多样性的病毒，能够特异性感染分枝杆菌属，包括结核分枝杆菌和耻垢分枝杆菌。在这里，我们通过使用耻垢分枝杆菌 mc 155 作为宿主，从中国内蒙古呼和浩特的土壤中分离并注释了一株温和型分枝杆菌噬菌体 Lang 的基因组。它属于有尾噬菌体目长尾科，通过透射电子显微镜确定。详细考虑了噬菌体的形态特征和某些生物学特性。噬菌体 Lang 的基因组长 41487bp，GC 含量为 66.85%，编码 60 个推定的开放阅读框，属于 G1 亚群。基因组注释表明，结构蛋白、组装蛋白、宿主复制/转录和裂解的基因存在于功能簇中。噬菌体 Lang 的基因组序列与分枝杆菌噬菌体 Grizzly 和 Sweets 的相似度超过 95%，在 Lang 中存在取代、插入和缺失的差异。一步生长曲线显示，Lang 的潜伏期为 30 分钟，爆发期为 90 分钟。潜伏期短和爆发期快标志着噬菌体 Lang 的独特特性，它可以成为对抗结核分枝杆菌的另一个潜在来源。
