Jia Fengjuan, Liu Xuecheng, Gong Zhiqing, Cui Wenjia, Wang Yansheng, Wang Wenliang
Institute of Agro-Food Science and Technology Shandong Academy of Agricultural Sciences Jinan Shandong PR China.
Key Laboratory of Agro-Products Processing Technology of Shandong Province Jinan Shandong PR China.
Food Sci Nutr. 2020 Sep 27;8(11):6131-6143. doi: 10.1002/fsn3.1905. eCollection 2020 Nov.
Dietary fiber (DF) has gained a great attention owing to its potential health benefits. is an edible fungus with high protein and low fat contents, which is also an enriched source of DF. However, limited study has been conducted on optimizing the conditions of . -derived DF extraction and modification as well as characterizing its properties. In this study, ultrasound-assisted enzymatic method for DF extraction was optimized as the following conditions: liquid material ratio of 29 ml/g, α-amylase concentration of 1.50%, protamex concentration of 1.20%, and ultrasonic power of 150 W, which improved the DF extraction yield to 37.70%. Moreover, high temperature modification (HTM) and cellulase modification (CEM) were applied to modify . -derived DF. The results showed that HTM had more potential capacity in converting insoluble DF into soluble DF, and DF with HTM exhibited more advantages in its physicochemical properties than DF with CEM. The DF with both HTM and CEM showed antioxidant activities, reflected by the increased reducing power as well as DPPH radical, hydroxyl radical, and ABTS scavenging capabilities in vitro. These findings could offer a reference for the extraction, modification, and characterizing various properties of DF from . , which would establish the foundation for the comprehensive application of fungi-derived DF.
膳食纤维（DF）因其潜在的健康益处而备受关注。[某种真菌名称]是一种高蛋白、低脂肪的食用菌，也是DF的丰富来源。然而，关于优化[某种真菌名称]衍生DF的提取和改性条件以及表征其特性的研究有限。在本研究中，优化了超声辅助酶法提取DF的条件如下：液料比为29 ml/g，α-淀粉酶浓度为1.50%，中性蛋白酶浓度为1.20%，超声功率为150 W，这使得DF提取率提高到37.70%。此外，采用高温改性（HTM）和纤维素酶改性（CEM）对[某种真菌名称]衍生的DF进行改性。结果表明，HTM在将不溶性DF转化为可溶性DF方面具有更大的潜力，并且经HTM改性的DF在理化性质上比经CEM改性的DF更具优势。经HTM和CEM改性的DF均表现出抗氧化活性，体外还原力增强以及对DPPH自由基、羟基自由基和ABTS的清除能力提高证明了这一点。这些发现可为从[某种真菌名称]中提取、改性DF并表征其各种特性提供参考，这将为真菌衍生DF的综合应用奠定基础。
