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具有抗sp.菌抑制作用及抗氧化能力的叶片多酚的微波辅助提取优化

Optimization of microwave assisted extraction of leaf polyphenols with inhibitory action against sp. and antioxidant capacity.

作者信息

Aguilar-Veloz Laura Maryoris, Calderón-Santoyo Montserrat, Ragazzo-Sánchez Juan Arturo

机构信息

Laboratorio Integral de Investigación en Alimentos, Tecnológico Nacional de México/Instituto Tecnológico de Tepic, Av. Tecnológico #2595, Col. Lagos del Country, C.P. 63175 Tepic, Nayarit México.

出版信息

Food Sci Biotechnol. 2021 Nov 21;30(13):1695-1707. doi: 10.1007/s10068-021-00996-8. eCollection 2021 Dec.

Abstract

The extracts are receiving attention due to their agro-food applications. Then, the simultaneous optimization of microwave-assisted extraction of polyphenols from jackfruit leaf with growth inhibitory action against sp. was studied. The effects of power and time on total soluble polyphenols and total flavonoids contents, and antifungal activity were investigated using response surface methodology. Temperature behavior was considered also. Models showed good prediction and successfully validation. Treatment at 840 W and 2 min allowed the responses maximization (148.75 mg galic acid equivalent /g dried weight of total soluble polyphenols, 13.28 mg rutin equivalent /g dried weight of total flavonoids, and 39.9% of antifungal activity). Furthermore, high ABTS (97%) and DPPH (92%) inhibition was exhibited, as a function of the polyphenol's concentration and composition. Mainly flavonoids with potential antioxidant and antifungal properties were detected. These findings suggest the potentialities of these extracts for sp. control during tomato postharvest.

摘要

由于其在农业食品领域的应用,这些提取物受到了关注。然后,研究了同时优化微波辅助从菠萝蜜叶中提取具有对某种菌生长抑制作用的多酚的方法。采用响应面法研究了功率和时间对总可溶性多酚和总黄酮含量以及抗真菌活性的影响。同时也考虑了温度变化情况。模型显示出良好的预测能力并成功得到验证。在840瓦和2分钟的处理条件下,各项响应指标达到最大值(总可溶性多酚为148.75毫克没食子酸当量/克干重,总黄酮为13.28毫克芦丁当量/克干重,抗真菌活性为39.9%)。此外,随着多酚浓度和组成的变化,提取物对ABTS(97%)和DPPH(92%)表现出较高的抑制率。检测到主要是具有潜在抗氧化和抗真菌特性的黄酮类化合物。这些发现表明这些提取物在番茄采后对某种菌的控制方面具有潜力。

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