Leoney Andonissamy, Karthigeyan Suma, Asharaf Ali Seyed, Felix A J W
Department of Prosthodontics, Rajah Muthiah Medical College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
Department of Community Medicine, Rajah Muthiah Medical College and Hospital, Annamalai University, Chidambaram, Tamil Nadu, India.
J Int Soc Prev Community Dent. 2020 Sep 28;10(5):627-633. doi: 10.4103/jispcd.JISPCD_256_20. eCollection 2020 Sep-Oct.
Complete denture patients have a plethora of microorganisms inhabiting their complete dentures. Some bacteria are capable of causing systemic illness such as aspiration pneumonia and infective endocarditis. Hence, detection as well as the categorization of biofilms, which form on the denture surface is vital in the study of denture biofilm-associated local and systemic diseases. This study aimed at the detection and categorization of biofilm-forming , , , and isolated from complete dentures and visualization of biofilms using scanning electron microscopy (SEM).
Thirty complete denture patients were selected for the study and swabs were collected from their complete denture surfaces. Isolation of the bacteria was done using selective media and confirmed using biochemical tests and 16SrRNA sequencing. The bacteria were subjected to biofilm assays via Microtiter plate assay. The biofilm-forming bacteria were categorized as weak, moderate, and strong biofilm formers based on optical density (OD) values. As a visual confirmation of the biofilms, scanning electron microscopic (SEM) images were taken for each of the strong biofilm-forming bacteria. Descriptive statistical analysis was carried out with the help of Statistical Package for the Social Sciences (SPSS) statistical package version 20.0.
The average OD of was 1. 333±0. 015 and the average OD of species was 1. 304 ± 0.023. The average OD value of was 0.8 ± 0.012 and the average value of was 1.014 ± 0.01.
The study of biofilms especially the strongly biofilm formers is very useful to understand the potential pathogenic effect of biofilms. These biofilms cause the systemic spread of the planktonic bacteria which could lead to systemic diseases that are resistant to conventional treatment. This could be due to the inherent nature of the biofilm to offer drug resistance to existing antibacterial agents.
全口义齿患者的全口义齿上栖息着大量微生物。一些细菌能够引发全身疾病，如吸入性肺炎和感染性心内膜炎。因此，义齿表面形成的生物膜的检测和分类对于研究义齿生物膜相关的局部和全身疾病至关重要。本研究旨在检测和分类从全口义齿中分离出的形成生物膜的[具体细菌种类1]、[具体细菌种类2]、[具体细菌种类3]和[具体细菌种类4]，并使用扫描电子显微镜（SEM）对生物膜进行可视化。
选择30名全口义齿患者进行研究，从他们的全口义齿表面采集拭子。使用选择性培养基分离细菌，并通过生化试验和16SrRNA测序进行确认。通过微量滴定板试验对细菌进行生物膜检测。根据光密度（OD）值将形成生物膜的细菌分为弱、中、强生物膜形成菌。作为生物膜的视觉确认，对每种强生物膜形成菌拍摄扫描电子显微镜（SEM）图像。借助社会科学统计软件包（SPSS）20.0版进行描述性统计分析。
[具体细菌种类1]的平均OD值为1.333±0.015，[具体细菌种类2]的平均OD值为1.304±0.023。[具体细菌种类3]的平均OD值为0.8±0.012，[具体细菌种类4]的平均值为1.014±0.01。
生物膜的研究，尤其是强生物膜形成菌的研究，对于理解生物膜的潜在致病作用非常有用。这些生物膜导致浮游细菌的全身传播，这可能导致对传统治疗有抗性的全身疾病。这可能是由于生物膜对现有抗菌剂具有耐药性的固有特性。
