Ruiz-Riaguas Alba, Fernández-de Córdova M L, Llorent-Martínez Eulogio J
Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Jaén, Spain.
Nat Prod Res. 2022 Jul;36(13):3445-3449. doi: 10.1080/14786419.2020.1855641. Epub 2020 Dec 7.
The aim of this study was to characterise the phenolic profile and evaluate the antioxidant activity of aqueous and methanol leaf extracts from Thunb., a species of flowering plant in the family. Liquid chromatography (HPLC) with diode array and electrospray-tandem mass spectrometry (DAD-ESI-MS) detection was used to characterise and quantify phenolic compounds. Thirty-two compounds were detected; among them, flavonoids (mainly quercetin and kaempferol glycosides) were the most abundant compounds in both extracts. The results obtained for DPPH and ABTS assays in the extracts were in agreement with the highest content of phenolics in the methanol extract.
本研究的目的是对来自[具体植物名称]（[植物所属科名]的一种开花植物）的叶片水提取物和甲醇提取物的酚类成分进行表征，并评估其抗氧化活性。采用配有二极管阵列和电喷雾串联质谱检测的液相色谱法（HPLC-DAD-ESI-MS）对酚类化合物进行表征和定量。共检测到32种化合物；其中，黄酮类化合物（主要是槲皮素和山奈酚糖苷）是两种提取物中含量最高的化合物。提取物的DPPH和ABTS分析结果与甲醇提取物中酚类物质的最高含量一致。
