虾青素生物合成基因的协调表达以提高虾青素产量。（原文最后少了具体物种或载体等信息，翻译只能到此为止）

Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in .

作者信息

Gong Zhongkuo, Wang Honglei, Tang Jinlei, Bi Changhao, Li Qingyan, Zhang Xueli

机构信息

School of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, China.

Tianjin Institute of Industrial Biotechnology, Chinese of Academy of Sciences, Tianjin 300308, China.

出版信息

J Agric Food Chem. 2020 Dec 16;68(50):14917-14927. doi: 10.1021/acs.jafc.0c05379. Epub 2020 Dec 8.

DOI:10.1021/acs.jafc.0c05379

PMID:33289384

Abstract

Astaxanthin has great potential commercial value in the feed, cosmetics, and nutraceutical industries due to its strong antioxidant capacity. In this study, the strain CAR026 with completely balanced metabolic flow was selected as the starting strain for the production of astaxanthin. The expression of β-carotene ketolase (CrtW) and β-carotene hydroxylase (CrtZ), which catalyze the conversion of β-carotene to astaxanthin, was coordinated, and a bottleneck was eliminated by increasing the copy number of in CAR026. The resulting strain Ast007 produced 21.36 mg/L and 4.6 mg/g DCW of astaxanthin in shake flasks. In addition, the molecular chaperone genes - were regulated to further improve the astaxanthin yield. The best strain Gro-46 produced 26 mg/L astaxanthin with a yield of 6.17 mg/g DCW in shake flasks and 1.18 g/L astaxanthin after 60 h of fermentation under fed-batch conditions. To the best of our knowledge, this is the highest astaxanthin obtained using engineered to date.

摘要

由于虾青素具有强大的抗氧化能力，它在饲料、化妆品和营养保健品行业具有巨大的潜在商业价值。在本研究中，选择代谢流完全平衡的CAR026菌株作为生产虾青素的出发菌株。对催化β-胡萝卜素转化为虾青素的β-胡萝卜素酮酶（CrtW）和β-胡萝卜素羟化酶（CrtZ）的表达进行了协调，并通过增加CAR026中[此处原文缺失相关基因名称]的拷贝数消除了一个瓶颈。所得菌株Ast007在摇瓶中产生了21.36 mg/L的虾青素和4.6 mg/g DCW（干重）的虾青素。此外，对分子伴侣基因进行调控以进一步提高虾青素产量。最佳菌株Gro-46在摇瓶中产生了26 mg/L的虾青素，产量为6.17 mg/g DCW，在补料分批培养条件下发酵60小时后产生了1.18 g/L的虾青素。据我们所知，这是迄今为止使用工程化[此处原文缺失相关微生物名称]获得的最高虾青素产量。