Department of Dental and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy.
Department of Surgical Sciences, Oral Biotechnology Laboratory, University of Cagliari, 09124 Cagliari, Italy.
Int J Environ Res Public Health. 2020 Dec 6;17(23):9106. doi: 10.3390/ijerph17239106.
(1) Background: Ozone (O3) proved to oxidize organic and inorganic compounds, and its efficacy against bacteria, viruses and fungi plasma membranes was of interest. Ozone vehicle can be a gaseous form, ozonated water or ozonized oil. The aim of this in-vitro study was to evaluate the efficacy of ozonated olive oil against . (2) Methods: Two different commercial mouthwashes were tested: Ialozon Blu (IB) (Gemavip, Cagliari, Italy), with ozonated olive oil, and Ialozon Rose (IR) (Gemavip, Cagliari, Italy), with ozonated olive oil, hyaluronic acid and vitamin E. All formulates were analyzed in a dilution range from 2- to 256-folds in saline solution, as to reproduce the salivary dilution. CIP103220 strain was used for the antimicrobial susceptibility test, and the Kirby-Bauer inhibition method was performed to evaluate the Minimum Inhibitory (MIC), Minimum Bactericidal (MBC), and Minimum Biofilm Inhibitory Concentration (MBIC). (3) Results: Both formulates showed the same antimicrobial activity. MIC, MBC, and MBIC were observed for dilution factors of 1/32, 1/8 and 1/8, respectively. The mean value of inhibition zone diameter was 16.5 mm for IB, and 18 mm for IR. (4) Conclusions: The results suggested that ozonized olive oil formulates were able to inactivate avoiding the salivary dilution effect in the oral cavity.
（1）背景：臭氧（O3）已被证明可氧化有机和无机化合物，其对细菌、病毒和真菌的细胞膜的功效引起了人们的兴趣。臭氧的载体可以是气态的，也可以是臭氧水或臭氧油。本体外研究旨在评估臭氧橄榄油对. 的功效。
（2）方法：测试了两种不同的商业漱口水：Ialozon Blu（IB）（Gemavip，卡利亚里，意大利），含有臭氧橄榄油，以及 Ialozon Rose（IR）（Gemavip，卡利亚里，意大利），含有臭氧橄榄油、透明质酸和维生素 E。所有配方均在生理盐水稀释范围内进行 2-256 倍稀释，以模拟唾液稀释。使用 CIP103220 菌株进行抗菌药敏试验，采用 Kirby-Bauer 抑制法评估最小抑菌浓度（MIC）、最小杀菌浓度（MBC）和最小生物膜抑制浓度（MBIC）。
（3）结果：两种配方均显示出相同的抗菌活性。MIC、MBC 和 MBIC 分别在 1/32、1/8 和 1/8 的稀释因子下观察到。IB 的抑菌圈直径平均值为 16.5mm，IR 为 18mm。
（4）结论：结果表明，臭氧橄榄油配方能够灭活 ，避免了口腔中唾液稀释的影响。
