鲜椰油和橄榄油混合物对口腔原发性定植菌的生物学活性：一项研究。

Biological Activities of Virgin Coconut and Virgin Olive Oil Mixture against Oral Primary Colonizers: An Study.

机构信息

Department of Children's Dentistry, Ministry of Health, Government of Malaysia, Kuala Lumpur, Malaysia, Orcid: https://orcid.org/0009-0005-9605-8788.

Department of Family Oral Health, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia, Phone: +60 166606493, e-mail:

出版信息

J Contemp Dent Pract. 2024 Mar 19;25(3):260-266. doi: 10.5005/jp-journals-10024-3645.

DOI:10.5005/jp-journals-10024-3645

PMID:38690700

Abstract

AIM AND BACKGROUND

This study aimed to explore the potential synergistic interaction of virgin coconut oil (VCO) and virgin olive oil (VOO) mixture against , , and in a single and mixture species through the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), antiadherence, and antibiofilm activities.

MATERIALS AND METHODS

The broth microdilution technique was used to individually determine the MIC of both oils and an oil mixture (in the ratio of 1:1) in a 96-well microtiter plate. As for the MBC, the subcultured method was used. The fractional inhibitory concentration index (ΣFIC) was determined to identify the interaction types between both oils. The oil mixture at its MIC was then tested on its antibiofilm and antiadherence effect.

RESULTS

The MIC of the oil mixture against the tested microbiota was 50-100%. The oil mixture was bactericidal at 100% concentration for all the mentioned microbes except . The ΣFIC value was 2 to 4, indicating that the VCO and VOO acted additively against the microbiota. Meanwhile, the oil mixture at MIC (50% for and ; 100% for and mixture species) exhibited antiadherence and antibiofilm activity toward the microbiota in mixture species.

CONCLUSION

The oil mixture possesses antibacterial, antibiofilm, and antiadherence properties toward the tested microbiota, mainly at 50-100% concentration of oil mixture. There was no synergistic interaction found between VCO and VOO.

CLINICAL SIGNIFICANCE

Children and individuals with special care may benefit from using the oil mixture, primarily to regulate the biofilm formation and colonization of the bacteria. Furthermore, the oil mixture is natural and nontoxic compared to chemical-based oral healthcare products. How to cite this article: Ng YM, Sockalingam SNMP, Shafiei Z, . Biological Activities of Virgin Coconut and Virgin Olive Oil Mixture against Oral Primary Colonizers: An Study. J Contemp Dent Pract 2024;25(3):260-266.

摘要

目的和背景

本研究旨在通过最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、抗黏附性和抗生物膜活性，探索初榨椰子油（VCO）和初榨橄榄油（VOO）混合物对单一和混合物种中、、和的潜在协同相互作用。

材料和方法

使用肉汤微量稀释技术在 96 孔微量滴定板中分别单独确定两种油和油混合物（比例为 1:1）的 MIC。对于 MBC，使用了亚培养方法。确定部分抑菌浓度指数（ΣFIC）以确定两种油之间的相互作用类型。然后，在 MIC 下测试油混合物对生物膜和抗黏附作用的影响。

结果

油混合物对测试菌群的 MIC 为 50-100%。油混合物在 100%浓度下对所有提到的微生物都是杀菌的，除了。ΣFIC 值为 2 至 4，表明 VCO 和 VOO 对菌群起相加作用。同时，在 MIC（50%用于和；100%用于和混合物种）下的油混合物对混合物种中的菌群表现出抗黏附性和抗生物膜活性。

结论

油混合物对测试菌群具有抗菌、抗生物膜和抗黏附特性，主要在 50-100%浓度的油混合物下。VCO 和 VOO 之间没有协同相互作用。

临床意义

儿童和特殊护理人群可能受益于使用油混合物，主要是为了调节细菌的生物膜形成和定植。此外，与基于化学的口腔保健产品相比，油混合物是天然无毒的。

如何引用本文

Ng YM, Sockalingam SNMP, Shafiei Z,. Biological Activities of Virgin Coconut and Virgin Olive Oil Mixture against Oral Primary Colonizers: An Study. J Contemp Dent Pract 2024;25(3):260-266.