Nakamura Yumi, Tsuchiya Takatsugu, Hara-Chikuma Mariko, Yasui Masato, Fukui Yuko
Suntory Global Innovation Center Ltd. Research Institute, 8-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto, 619-0284, Japan.
Department of Pharmacology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku, Tokyo, 160-8582, Japan.
Biochem Biophys Rep. 2020 Nov 23;24:100864. doi: 10.1016/j.bbrep.2020.100864. eCollection 2020 Dec.
In a previous clinical study, the moisture content in the stratum corneum of healthy Japanese women who consumed a beverage rich in oligomeric proanthocyanidins (OPCs) made from red wine extract was found to be higher than that in the control group. This finding suggested that OPCs can increase skin moisture content. In this study, we determined the expression level of aquaporin-3 (AQP3) in keratinocytes to elucidate the mechanism by which compounds in red wine grape increase moisture content in stratum corneum. Through in vitro studies, we confirmed that normal human epidermal keratinocytes (NHEK) incubated with red wine induced expression. Furthermore, the supplementation of red wine fractions enriched in OPC was shown to increase expression. Besides, the component of OPC-rich fractions that upregulated AQP3 expression was found to be a gallic acid (GA)-binding flavan-3-ol, particularly oligomeric compounds. We found that GA-binding OPC were able to upregulate expression and that these compounds were enriched in red wine. Our findings might suggest that the mechanism of enhancement of moisture content in stratum corneum by red wine might be via the upregulation of expression in the epidermal keratinocytes.
在之前的一项临床研究中，发现饮用富含由红酒提取物制成的低聚原花青素（OPC）饮料的健康日本女性角质层中的水分含量高于对照组。这一发现表明OPC可以增加皮肤水分含量。在本研究中，我们测定了角质形成细胞中水通道蛋白3（AQP3）的表达水平，以阐明红酒葡萄中的化合物增加角质层水分含量的机制。通过体外研究，我们证实用红酒培养的正常人表皮角质形成细胞（NHEK）诱导了表达。此外，添加富含OPC的红酒组分可增加表达。此外，发现上调AQP3表达的富含OPC组分的成分是没食子酸（GA）结合的黄烷-3-醇，特别是低聚化合物。我们发现GA结合的OPC能够上调表达，并且这些化合物在红酒中含量丰富。我们的研究结果可能表明，红酒增强角质层水分含量的机制可能是通过上调表皮角质形成细胞中的表达。
