Sánchez-Alonzo Kimberly, Parra-Sepúlveda Cristian, Vergara Lorena, Bernasconi Humberto, García-Cancino Apolinaria
Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas, región del Biobío, Chile.
Departamento de Odontología Restauradora, Facultad de Odontología. Universidad de Concepción, Concepción, Chile.
Rev Assoc Med Bras (1992). 2020 Nov;66(11):1509-1514. doi: 10.1590/1806-9282.66.11.1509.
Nearly 73% of the Chilean population is infected with Helicobacter pylori (H. pylori), a factor predisposing for gastric cancer. Recent studies have demonstrated the presence of this pathogen within yeasts, suggesting that this fact can directly influence the failure of a treatment, transmission, and reinfection.
To detect the presence of H. pylori inside oral yeasts isolated from students of the University of Concepción (Chile).
72 samples, obtained from the oral cavity using cotton swabs were incubated in YPD broth for 48h at 37°C and posteriorly seeded in Sabouraud Dextrose agar plus chloramphenicol at the same temperature and for the same time. Yeasts isolated were observed microscopically (wet mounting and Gram-stained) and identified using microbiological techniques. Intracellular H. pylori detection was performed by the amplification of 16S rDNA by PCR.
Oral yeasts were detected in 24 samples (33.3%), being C. albicans (79.2%) the most frequent species, followed by C. dubliniensis (12.4%), C. krusei (4.2%), and C. tropicalis (4.2%). When analyzed by PCR, 15 of the 24 oral yeasts 62.5 % were positive for H. pylori 16S rDNA. From the 15 individuals positive for yeast harboring H. pylori, 81% of them reported stomach discomfort, and the presence of the bacteria was diagnosed at some moment in 20% of them.
The intracellular presence of the H. pylori in oral yeasts suggests an endosymbiotic relationship of these microorganisms, which could favor H. pylori transmission and reinfection in the gastrointestinal tract.
近73%的智利人口感染幽门螺杆菌（H. pylori），这是胃癌的一个诱发因素。最近的研究表明，这种病原体存在于酵母中，这表明这一事实可能直接影响治疗失败、传播和再感染。
检测从康塞普西翁大学（智利）学生中分离出的口腔酵母内幽门螺杆菌的存在情况。
用棉签从口腔获取72份样本，在YPD肉汤中于37°C孵育48小时，然后在相同温度和时间下接种于含氯霉素的沙氏葡萄糖琼脂培养基上。对分离出的酵母进行显微镜观察（湿片法和革兰氏染色），并使用微生物学技术进行鉴定。通过PCR扩增16S rDNA来检测细胞内幽门螺杆菌。
在24份样本（33.3%）中检测到口腔酵母，其中白色念珠菌（79.2%）是最常见的菌种，其次是都柏林念珠菌（12.4%）、克鲁斯念珠菌（4.2%）和热带念珠菌（4.2%）。通过PCR分析，24份口腔酵母样本中有15份（62.5%）幽门螺杆菌16S rDNA呈阳性。在15例携带幽门螺杆菌的酵母阳性个体中，81%报告有胃部不适，其中20%在某个时候被诊断出存在该细菌。
口腔酵母内存在细胞内幽门螺杆菌表明这些微生物存在内共生关系，这可能有利于幽门螺杆菌在胃肠道的传播和再感染。
