State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Institute of Plant Protection, Henan Academy of Agricultural Sciences/Key Laboratory of IPM of Pests on Crop (Southern North China), Ministry of Agriculture, Key Laboratory of Crop Pest Control of Henan, Zhengzhou, Henan 450002, China.
Mol Plant Microbe Interact. 2021 Apr;34(4):426-438. doi: 10.1094/MPMI-11-20-0304-R. Epub 2021 Mar 24.
Thioredoxins (Trxs) function within the antioxidant network through modulation of one or more redox reactions involved in oxidative-stress signaling. Given their function in regulating cellular redox, Trx proteins also fulfill key roles in plant immune signaling. Here, , encoding a subgroup member of the Trx family, was identified and cloned in wheat (), which was rapidly induced by f. sp. invasion and salicylic acid (SA) treatment. Overexpression of in tobacco () induced programmed cell death. Silencing of in wheat enhanced susceptibility to f. sp. in different aspects, including reactive oxygen species accumulation and pathogen-responsive or -related gene expression. Herein, we observed that the cellular concentration of SA was significantly reduced in -silenced plants, indicating that possibly regulates wheat resistance to stripe rust through a SA-associated defense signaling pathway. Using a yeast two-hybrid screen to identify TaTrxh1-interacting partners, we further show that interaction with TaCP1 (a RD19-like cysteine protease) and subsequent silencing of reduced wheat resistance to f. sp. . In total, the data presented herein demonstrate that enhances wheat resistance against f. sp. via SA-dependent resistance signaling and that TaTrxh1 interaction with TaCP1 is required for wheat resistance to stripe rust.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
硫氧还蛋白（Trxs）通过调节氧化应激信号转导中涉及的一个或多个氧化还原反应，在抗氧化网络中发挥作用。鉴于其在调节细胞氧化还原中的功能，Trx 蛋白在植物免疫信号转导中也发挥着关键作用。在这里，鉴定并克隆了小麦（Triticum aestivum）中的一种 Trx 家族亚组 成员（编码基因），该基因在 条锈菌（Puccinia striiformis f. sp. tritici）侵染和水杨酸（SA）处理后迅速诱导表达。烟草（Nicotiana tabacum）中 的过表达诱导程序性细胞死亡。小麦中 的沉默在多个方面增强了对 条锈菌的敏感性，包括活性氧物质的积累以及与病原体相关或相关基因的表达。在此，我们观察到沉默植株中 SA 的细胞浓度显著降低，表明 通过与 SA 相关的防御信号通路可能调节小麦对条锈病的抗性。通过酵母双杂交筛选鉴定 TaTrxh1 的互作伙伴，我们进一步表明 TaTrxh1 与 TaCP1（一种 RD19 样半胱氨酸蛋白酶）的相互作用以及随后 的沉默降低了小麦对 条锈菌的抗性。总之，本文数据表明， 通过依赖 SA 的抗性信号增强小麦对 条锈菌的抗性，而 TaTrxh1 与 TaCP1 的相互作用是小麦抗条锈病所必需的。[公式：见正文]版权所有 © 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。
