Corteva Agriscience, Johnston, IA, USA.
Nat Plants. 2020 Dec;6(12):1427-1431. doi: 10.1038/s41477-020-00817-6. Epub 2020 Dec 7.
CRISPR-Cas is a powerful double-strand-break technology with wide-ranging applications from gene discovery to commercial product development. Thus far, this tool has been almost exclusively used for gene knockouts and deletions, with a few examples of gene edits and targeted gene insertions. Here, we demonstrate the application of CRISPR-Cas9 technology to mediate targeted 75.5-Mb pericentric inversion in chromosome 2 in one of the elite maize inbred lines from Corteva Agriscience. This inversion unlocks a large chromosomal region containing substantial genetic variance for recombination, thus providing opportunities for the development of new maize varieties with improved phenotypes.
CRISPR-Cas 是一种强大的双链断裂技术，在基因发现到商业产品开发等领域有着广泛的应用。迄今为止，该工具几乎专门用于基因敲除和缺失，只有少数基因编辑和靶向基因插入的例子。在这里，我们展示了 CRISPR-Cas9 技术在科迪华农业科学公司的一个优秀玉米自交系中 2 号染色体上介导靶向 75.5Mb 着丝粒倒位的应用。这种倒位解开了一个包含大量重组遗传变异的大型染色体区域，从而为开发具有改良表型的新型玉米品种提供了机会。
