Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, People's Republic of China.
School of Pharmacy, Harbin Medical University, Harbin 150081, People's Republic of China.
J Nat Prod. 2020 Dec 24;83(12):3635-3641. doi: 10.1021/acs.jnatprod.0c00808. Epub 2020 Dec 10.
Four new cyclodepsipeptides, trichodestruxins A-D (-), together with destruxin E2 chlorohydrin () and destruxin A2 (), were isolated from the plant endophytic fungus by a bioassay-guided fractionation method. Their planar structures were elucidated on the basis of 1D and 2D NMR and MS/MS spectroscopic analyses. The stereochemical configuration was established by application of the advanced Marfey's method, -based configuration analysis, Mosher's method, and chemical derivatizations. Trichodestruxin B contains hydroxy acid fragments of the THPA unit, while trichodestruxins A and C contain the β-methylproline moiety. All cyclodepsipeptides displayed cytotoxicity against HT-29, A549, and/or P388 cell lines with IC values of 0.7-19.1 μM.
四种新的环二肽，即 trichodestruxins A-D (-)，以及 destruxin E2 氯醇 () 和 destruxin A2 ()，从植物内生真菌 通过生物测定指导的分离方法分离得到。根据 1D 和 2D NMR 和 MS/MS 光谱分析，阐明了它们的平面结构。立体化学构型通过应用先进的 Marfey's 方法、基于的构型分析、Mosher's 方法和化学衍生化来确定。Trichodestruxin B 含有 THPA 单元的羟基酸片段，而 trichodestruxins A 和 C 含有β-甲基脯氨酸部分。所有环二肽都显示出对 HT-29、A549 和/或 P388 细胞系的细胞毒性，IC 值为 0.7-19.1 μM。
