来自内生真菌W8的细胞毒性和抗菌环缩肽
Cytotoxic and Antibacterial Cyclodepsipeptides from an Endophytic Fungus W8.
作者信息
Wang Zimo, Liu Bo, Wang Yanlei, Xu Yicen, Ma Hai, Sun Yi
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Molecules. 2024 Dec 5;29(23):5746. doi: 10.3390/molecules29235746.
Seven cyclic depsipeptides, including two new cyclic pentadepsipeptides avenamides A () and B (), were isolated from a plant-derived fungus W8 by using the bioassay-guided fractionation method. The planar structures were elucidated by using comprehensive spectroscopic analyses, including 1D and 2D NMR, as well as MS/MS spectrometry. The absolute configuration of the amino acid and hydroxy acid residues was confirmed by using the advanced Marfey's method and chiral HPLC analysis, respectively. Compounds - were evaluated for their cytotoxic activities against A549 and NCI-H1944 human lung adenocarcinoma cell lines and their antimicrobial activities against and . As a result, compounds - showed moderate cytotoxicity, with IC values of 6.5245.20 µM. Compounds and exhibited significant antimicrobial activities against and , with an MIC80 of 11.130.0 µg/mL.
通过生物活性导向分离法，从一株植物源真菌W8中分离出7种环缩肽，包括两种新的环五缩肽燕麦酰胺A（）和B（）。通过综合光谱分析，包括一维和二维核磁共振以及串联质谱，阐明了其平面结构。分别使用先进的马尔费方法和手性高效液相色谱分析确定了氨基酸和羟基酸残基的绝对构型。评估了化合物 - 对A549和NCI-H1944人肺腺癌细胞系的细胞毒性活性以及对 和 的抗菌活性。结果，化合物 - 表现出中等细胞毒性，IC值为6.5245.20 μM。化合物 和 对 和 表现出显著的抗菌活性，MIC80为11.130.0 μg/mL。
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