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NMR 代谢组学分析希腊蜂胶样品:比较其植物化学成分的评价及抗老化和抗氧化性能的研究。

NMR metabolic profiling of Greek propolis samples: Comparative evaluation of their phytochemical compositions and investigation of their anti-ageing and antioxidant properties.

机构信息

Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens, 11527, Greece.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens, 11527, Greece.

出版信息

J Pharm Biomed Anal. 2021 Feb 5;194:113814. doi: 10.1016/j.jpba.2020.113814. Epub 2020 Dec 2.

Abstract

The present study aimed to investigate the metabolic profile, as well as the antioxidant and anti-ageing activities of twenty propolis samples from different regions of Greece. Chemical profiling of methanolic extracts was investigated using HPTLC and H-NMR techniques. Their antioxidant activity was evaluated by free radical scavenging methods (DPPH and ABTS), whereas anti-ageing properties were assessed as anti-collagenase activity. Extracts were also investigated in vitro for their ability to inhibit tyrosinase, which is responsible for the oxidation of L-DOPA to dopachrome and the production of melanin. The HPTLC and NMR analysis revealed high variability in the phytochemical profile of the methanolic extracts, with three major groups to be observed: a) Group I, consisting of samples rich in terpenoids, which present low antioxidant but high anti-tyrosinase activity, b) Group II, consisting of samples rich in flavonoids, which form a broad cluster with major similarities at the aromatic region and showed the highest anti-oxidant and anti-collagenase activities and c) Group III, consisting of samples with lower flavonoid content than the samples of Group II, which exhibited moderate antioxidant, anti-collagenase and anti-tyrosinase activities. In conclusion, this study has shown high differentiation on the chromatographic and spectroscopic metabolic profile of Greek propolis samples of different geographical origin, which is also reflected in their biological properties. Their important effects as antioxidant, anti-tyrosinase and anti-collagenase agents make propolis an important potent ingredient in the industry of food supplements and cosmeceuticals. Moreover, a correlation of a particular chemical propolis type to a specific type of biological activity will allow to prepare standardized extracts and develop food supplements and cosmeceuticals possessing the desired pharmacological properties.

摘要

本研究旨在探讨二十种来自希腊不同地区的蜂胶样品的代谢特征,以及其抗氧化和抗衰老活性。采用 HPTLC 和 H-NMR 技术研究了甲醇提取物的化学特征。通过自由基清除方法(DPPH 和 ABTS)评估其抗氧化活性,通过抗胶原酶活性评估其抗衰老特性。还评估了提取物在体外抑制酪氨酸酶的能力,酪氨酸酶负责将 L-DOPA 氧化为多巴醌并产生黑色素。HPTLC 和 NMR 分析显示,甲醇提取物的植物化学成分谱具有高度的可变性,可观察到三个主要组:a)组 I,由富含萜烯的样品组成,其抗氧化活性低但抗酪氨酸酶活性高,b)组 II,由富含类黄酮的样品组成,这些样品在芳香区域具有主要相似性,形成一个广泛的簇,表现出最高的抗氧化和抗胶原酶活性,c)组 III,由类黄酮含量低于组 II 样品的样品组成,表现出中等强度的抗氧化、抗胶原酶和抗酪氨酸酶活性。总之,这项研究表明,不同地理来源的希腊蜂胶样品在色谱和光谱代谢特征上存在高度分化,这也反映在它们的生物学特性上。它们作为抗氧化剂、抗酪氨酸酶和抗胶原酶的重要作用使蜂胶成为食品补充剂和化妆品行业的重要有效成分。此外,将特定的化学蜂胶类型与特定类型的生物活性相关联,将允许制备标准化提取物并开发具有所需药理学特性的食品补充剂和化妆品。

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