Zhuravlev Evgenii, Sergeeva Mariia, Malanin Sergey, Amirkhanov Rinat, Semenov Dmitriy, Grigoryeva Tatiana, Komissarov Andrey, Stepanov Grigory
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Smorodintsev Research Institute of Influenza, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
Data Brief. 2020 Nov 29;33:106604. doi: 10.1016/j.dib.2020.106604. eCollection 2020 Dec.
Human influenza remains a serious public health problem. This data article reports the transcriptome analysis data of human cell lines infected with influenza A/Puerto Rico/8/1934 (H1N1) virus. Mock-infected cells were included as controls. Human embryonic fibroblasts (MRC-5) and immortalized cell lines (A549, HEK293FT, WI-38 VA-13) were selected for RNA sequencing using Illumina NextSeq500 platform. Raw data were applied to the bioinformatic pipeline, which includes quality control with FastQC and MultiQC, adapter and quality trimming with Cutadapt, filtering to the genome of influenza A with STAR, transcript quantification with Salmon tool (GRCh38_RefSeq_Transcripts). Differential expressed genes were identified using R package DESeq2 with FDR-adjusted p-value < 0.001 and absolute value of log2(FC) > 1. Lists of differentially expressed genes is provided. The raw and processed RNA-seq data presented in this article were deposited to the European Nucleotide Archive via the ArrayExpress partner repository with the dataset accession number E-MTAB-9511 .
人类流感仍然是一个严重的公共卫生问题。这篇数据文章报告了感染甲型流感病毒/波多黎各/8/1934（H1N1）的人类细胞系的转录组分析数据。以 mock 感染的细胞作为对照。选择人类胚胎成纤维细胞（MRC-5）和永生化细胞系（A549、HEK293FT、WI-38 VA-13），使用 Illumina NextSeq500 平台进行 RNA 测序。原始数据应用于生物信息学流程，该流程包括使用 FastQC 和 MultiQC 进行质量控制、使用 Cutadapt 进行接头和质量修剪、使用 STAR 过滤到甲型流感病毒基因组、使用 Salmon 工具（GRCh38_RefSeq_Transcripts）进行转录本定量。使用 R 包 DESeq2 鉴定差异表达基因，FDR 校正的 p 值 < 0.001 且 log2（FC）的绝对值 > 1。提供了差异表达基因列表。本文中呈现的原始和处理后的 RNA-seq 数据通过 ArrayExpress 合作伙伴存储库存入欧洲核苷酸档案馆，数据集登录号为 E-MTAB-9511。
