Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, PR China.
Key Laboratory of Polar Science, MNR, Polar Research Institute of China, Shanghai, 200136, PR China.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004605. Epub 2020 Dec 11.
A Gram-negative, aerobic, rod-shaped, non-motile bacterium, designated strain HQ09, was isolated from a marine sponge off the coast of Fields Peninsula, West Antarctica. Strain HQ09 grew at 4-35 °C (optimum, 25 °C), pH 5-9 (optimum, pH 7.0), and with 1-10% NaCl (optimum, 2 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain HQ09 was affiliated with the genus in the family , sharing 99.64 % identity with the type strain of , the only known species in the genus. However, the low digital DNA-DNA hybridization (dDDH) (27.2 %) and average nucleotide identity (ANI) (83.63 %) values between strain HQ09 and the type strain of indicated that they did not belong to the same species. Strain HQ09 could also be differentiated from by many phenotypic characteristics. The major fatty acids (>5 %) of strain HQ09 were summed feature 8 (C C ω6c), 11-methyl C c, C and C19 : 0 cyclo 8. The polar lipids included phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids and one unidentified phospholipid. The predominant respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content was 62.63 mol%. Four secondary metabolite biosynthetic gene clusters were detected in the genome, potentially producing ectoine and three types of unknown compounds. On the basis of the polyphasic evidences obtained in this study, strain HQ09 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain being HQ09 (=KCTC 52229=CGMCC 1.15538).
一株革兰氏阴性、需氧、杆状、非运动细菌，被命名为 HQ09 株，从南极洲西部菲尔德斯半岛海域的海绵中分离得到。HQ09 株在 4-35°C（最适温度 25°C）、pH5-9（最适 pH7.0）和 1-10%NaCl（最适 2%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，HQ09 株与属内的属相关，与属内唯一已知种的模式菌株的同源性为 99.64%。然而，HQ09 株与模式菌株的 DNA-DNA 杂交（dDDH）值（27.2%）和平均核苷酸同一性（ANI）值（83.63%）较低，表明它们不属于同一物种。HQ09 株还可以通过许多表型特征与属区分开来。HQ09 株的主要脂肪酸（>5%）为 summed feature 8（C Cω6c）、11-甲基 C c、C 和 C19：0 cyclo 8。极性脂包括磷脂酰甘油、磷脂酰胆碱、两种未鉴定的氨基脂和一种未鉴定的磷脂。主要的呼吸醌为泛醌 10（Q-10）。基因组 DNA G+C 含量为 62.63mol%。在基因组中检测到四个次级代谢产物生物合成基因簇，可能产生海藻糖和三种类型的未知化合物。基于本研究获得的多相证据，HQ09 株代表了属的一个新种，建议采用新种名 sp. nov.，其模式菌株为 HQ09（=KCTC 52229=CGMCC 1.15538）。
