• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

构建针对Ⅱ型胶原的 CAR-T 细胞，以探索软骨反应性 T 细胞与软骨细胞之间的细胞因子级联反应。

Construction of CII-Specific CAR-T to Explore the Cytokine Cascades Between Cartilage-Reactive T Cells and Chondrocytes.

机构信息

Central Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.

College of Life Science, Northeast Agricultural University, Harbin, China.

出版信息

Front Immunol. 2020 Dec 4;11:568741. doi: 10.3389/fimmu.2020.568741. eCollection 2020.

DOI:10.3389/fimmu.2020.568741
PMID:33343563
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7746615/
Abstract

Cytokine cascades exist in many autoimmune disorders which amplify and sustain the autoimmune process and lead to chronic inflammatory injury to the host tissues. Increasing evidence indicates that chondrocytes can interact with T cells, which may be a crucial event in inflammatory arthritis. To address the reciprocal influences of cartilage-reactive T cells and chondrocytes, we constructed cartilage-reactive T cells by developing a type II collagen-specific chimeric antigen receptor (CII-CAR). An co-culture model of CII-CAR-T cells and fresh cartilage was developed, in which CII-CAR-T displayed specific proliferative capacity and cytokine release against fresh cartilage samples, and chondrocytes could respond to CII-CAR-T cells by secreting IL-6. The proposed model will help us to explore the possible cytokine cascades between cartilage-reactive T cells and cartilage.

摘要

细胞因子级联反应存在于许多自身免疫性疾病中，这些级联反应放大并维持自身免疫过程，导致宿主组织的慢性炎症损伤。越来越多的证据表明，软骨细胞可以与 T 细胞相互作用，这可能是炎症性关节炎的一个关键事件。为了研究软骨反应性 T 细胞和软骨细胞之间的相互影响，我们通过构建 II 型胶原特异性嵌合抗原受体（CII-CAR）来构建软骨反应性 T 细胞。建立了 CII-CAR-T 细胞与新鲜软骨的共培养模型，该模型中 CII-CAR-T 细胞对新鲜软骨样本表现出特异性增殖能力和细胞因子释放，软骨细胞可以通过分泌 IL-6 对 CII-CAR-T 细胞做出反应。该模型将有助于我们探讨软骨反应性 T 细胞和软骨之间可能存在的细胞因子级联反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/45096fced27a/fimmu-11-568741-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/27c3e06a7fa2/fimmu-11-568741-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/937653e8eec3/fimmu-11-568741-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/d0b91989c6a8/fimmu-11-568741-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/297fd18dc402/fimmu-11-568741-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/df13a71daaf2/fimmu-11-568741-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/45096fced27a/fimmu-11-568741-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/27c3e06a7fa2/fimmu-11-568741-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/937653e8eec3/fimmu-11-568741-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/d0b91989c6a8/fimmu-11-568741-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/297fd18dc402/fimmu-11-568741-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/df13a71daaf2/fimmu-11-568741-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a3b/7746615/45096fced27a/fimmu-11-568741-g006.jpg

相似文献

1
Construction of CII-Specific CAR-T to Explore the Cytokine Cascades Between Cartilage-Reactive T Cells and Chondrocytes.构建针对Ⅱ型胶原的 CAR-T 细胞，以探索软骨反应性 T 细胞与软骨细胞之间的细胞因子级联反应。
Front Immunol. 2020 Dec 4;11:568741. doi: 10.3389/fimmu.2020.568741. eCollection 2020.
2
Allogeneic mesenchymal stem cell and mesenchymal stem cell-differentiated chondrocyte suppress the responses of type II collagen-reactive T cells in rheumatoid arthritis.同种异体间充质干细胞和间充质干细胞分化的软骨细胞可抑制类风湿关节炎中II型胶原反应性T细胞的反应。
Rheumatology (Oxford). 2008 Jan;47(1):22-30. doi: 10.1093/rheumatology/kem284.
3
A Shared Epitope of Collagen Type XI and Type II Is Recognized by Pathogenic Antibodies in Mice and Humans with Arthritis.关节炎小鼠和人类的致病性抗体识别 XI 型和 II 型胶原的共同表位。
Front Immunol. 2018 Apr 12;9:451. doi: 10.3389/fimmu.2018.00451. eCollection 2018.
4
Arthritogenic anti-type II collagen antibodies are pathogenic for cartilage-derived chondrocytes independent of inflammatory cells.致关节炎的抗II型胶原蛋白抗体对源自软骨的软骨细胞具有致病性，且不依赖于炎症细胞。
Arthritis Rheum. 2005 Jun;52(6):1897-906. doi: 10.1002/art.21097.
5
The major T cell epitope on type II collagen is glycosylated in normal cartilage but modified by arthritis in both rats and humans.II型胶原蛋白上的主要T细胞表位在正常软骨中是糖基化的，但在大鼠和人类中都会因关节炎而发生改变。
Eur J Immunol. 2005 Feb;35(2):357-66. doi: 10.1002/eji.200425637.
6
An arthritogenic monoclonal antibody to type II collagen, CII-C1, impairs cartilage formation by cultured chondrocytes.一种针对II型胶原蛋白的致关节炎单克隆抗体CII-C1，会损害培养的软骨细胞的软骨形成。
Immunol Cell Biol. 2004 Aug;82(4):427-34. doi: 10.1111/j.0818-9641.2004.01267.x.
7
T cells that are naturally tolerant to cartilage-derived type II collagen are involved in the development of collagen-induced arthritis.对软骨来源的II型胶原天然耐受的T细胞参与了胶原诱导性关节炎的发病过程。
Arthritis Res. 2000;2(4):315-26. doi: 10.1186/ar106. Epub 2000 Jun 5.
8
Cysteine proteases in Langerhans cells limits presentation of cartilage derived type II collagen for autoreactive T cells.朗格汉斯细胞中的半胱氨酸蛋白酶限制了自身反应性T细胞对软骨来源的II型胶原蛋白的呈递。
Int Immunol. 2004 May;16(5):717-26. doi: 10.1093/intimm/dxh079. Epub 2004 Apr 13.
9
Ex vivo characterization of the autoimmune T cell response in the HLA-DR1 mouse model of collagen-induced arthritis reveals long-term activation of type II collagen-specific cells and their presence in arthritic joints.在胶原诱导性关节炎的HLA - DR1小鼠模型中，对自身免疫性T细胞反应的体外特征分析揭示了II型胶原特异性细胞的长期激活及其在关节炎关节中的存在。
J Immunol. 2005 Apr 1;174(7):3978-85. doi: 10.4049/jimmunol.174.7.3978.
10
Specific antibody protection of the extracellular cartilage matrix against collagen antibody-induced damage.细胞外软骨基质的特异性抗体对胶原抗体诱导损伤的保护作用。
Arthritis Rheum. 2010 Nov;62(11):3374-84. doi: 10.1002/art.27671.

引用本文的文献

1
From spheroids to organoids: next-generation models for CAR-T cell therapy research in solid tumors.从球体到类器官：实体瘤中CAR-T细胞疗法研究的下一代模型
Front Immunol. 2025 Jul 11;16:1626369. doi: 10.3389/fimmu.2025.1626369. eCollection 2025.

本文引用的文献

1
Prospects of the Use of Cell Therapy to Induce Immune Tolerance.细胞治疗诱导免疫耐受的前景。
Front Immunol. 2020 May 12;11:792. doi: 10.3389/fimmu.2020.00792. eCollection 2020.
2
Dual Role of Chondrocytes in Rheumatoid Arthritis: The Chicken and the Egg.软骨细胞在类风湿关节炎中的双重作用：先有鸡还是先有蛋。
Int J Mol Sci. 2020 Feb 6;21(3):1071. doi: 10.3390/ijms21031071.
3
Peptidylarginine Deiminase Autoimmunity and the Development of Anti-Citrullinated Protein Antibody in Rheumatoid Arthritis: The Hapten-Carrier Model.
肽酰基精氨酸脱亚氨酶自身免疫与类风湿关节炎中抗瓜氨酸化蛋白抗体的发展：半抗原-载体模型。
Arthritis Rheumatol. 2020 Jun;72(6):903-911. doi: 10.1002/art.41189. Epub 2020 Apr 26.
4
LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis.LncRNA PICSAR 通过海绵吸附 miRNA-4701-5p 促进类风湿关节炎成纤维样滑膜细胞的增殖、迁移和侵袭。
EBioMedicine. 2019 Dec;50:408-420. doi: 10.1016/j.ebiom.2019.11.024. Epub 2019 Nov 30.
5
Role of Chondrocytes in the Development of Rheumatoid Arthritis Via Transmembrane Protein 147-Mediated NF-κB Activation.跨膜蛋白 147 介导 NF-κB 激活在软骨细胞致类风湿关节炎中的作用。
Arthritis Rheumatol. 2020 Jun;72(6):931-942. doi: 10.1002/art.41182. Epub 2020 May 2.
6
HLA associations in inflammatory arthritis: emerging mechanisms and clinical implications.炎症性关节炎中的 HLA 相关性：新出现的机制及临床意义。
Nat Rev Rheumatol. 2019 Jun;15(6):364-381. doi: 10.1038/s41584-019-0219-5.
7
Helios but not CD226, TIGIT and Foxp3 is a Potential Marker for CD4 Treg Cells in Patients with Rheumatoid Arthritis.Helios而非CD226、TIGIT和Foxp3是类风湿关节炎患者CD4调节性T细胞的潜在标志物。
Cell Physiol Biochem. 2019;52(5):1178-1192. doi: 10.33594/000000080.
8
T-cells interact with B cells, dendritic cells, and fibroblast-like synoviocytes as hub-like key cells in rheumatoid arthritis.T 细胞在类风湿关节炎中作为枢纽样关键细胞与 B 细胞、树突状细胞和成纤维样滑膜细胞相互作用。
Int Immunopharmacol. 2019 May;70:428-434. doi: 10.1016/j.intimp.2019.03.008. Epub 2019 Mar 8.
9
1,25-Dihydroxyvitamin D3 Ameliorates Collagen-Induced Arthritis via Suppression of Th17 Cells Through miR-124 Mediated Inhibition of IL-6 Signaling.1,25-二羟维生素 D3 通过 miR-124 介导的抑制 IL-6 信号通路抑制 Th17 细胞缓解胶原诱导性关节炎。
Front Immunol. 2019 Feb 7;10:178. doi: 10.3389/fimmu.2019.00178. eCollection 2019.
10
Differential roles of TNFα-TNFR1 and TNFα-TNFR2 in the differentiation and function of CD4Foxp3 induced Treg cells in vitro and in vivo periphery in autoimmune diseases.在自身免疫性疾病中，TNFα-TNFR1 和 TNFα-TNFR2 在体外和体内外周 CD4Foxp3 诱导的 Treg 细胞的分化和功能中发挥不同的作用。
Cell Death Dis. 2019 Jan 10;10(1):27. doi: 10.1038/s41419-018-1266-6.