Niu Jiang-Tao, Cao Rui, Si Xin-Lei, Bian Tian-Tian, Xin Er-Dan, Li Yue-Feng, Yan Xing-Ke
Pharmacy of College, Gansu University of Chinese Medicine, Lanzhou 730000, China.
Key Laboratory of Quality and Standard of TCM of Gansu Province, Lanzhou 730000, China.
Evid Based Complement Alternat Med. 2020 Dec 2;2020:4543761. doi: 10.1155/2020/4543761. eCollection 2020.
To compare the changes of chemical components of Hedysari Radix (HR) before and after honey-processing, and to explore the material basis of the difference between HR and honey-processed Hedysari Radix (HPHR) in Buzhong Yiqi.
Different compounds in aqueous extracts of HR and HPHR were analysed by UPLC-MS. A rat model of spleen qi deficiency was established. The rats were treated with different doses of water extracts of HR or HPHR, and pathological differences in spleen tissue, serum levels of -xylose, gastrin (GAS) and amylase (AMS) interleukin-2 (IL)-2 and tumour necrosis factor- (TNF-), as well as spleen and thymus indices, were used as indicators. Differences in the efficacy of HR and HPHR in Buzhong Yiqi were studied.
The research showed that compared with the blank group, the spleen tissue of rats in the model group showed spleen tissue damage, which mainly manifested as unclear boundaries between red pulp and white pulp, irregular spleen morphology and irregular arrangement, and the structure of white pulp destruction, less lymphocytes, the number of germinal centers decreased or atrophied. Compared with the model group, the middle and high dose groups of HR and HPHR had protective effects on spleen tissue of spleen-qi deficiency rats, and HPHR had a stronger effect; compared with those in the model group, rats in each treatment group showed remarkably higher serum -xylose, GAS and AMS levels and thymus and spleen indices, and remarkably lower serum IL-2 and TNF- levels, among which HPHR group showed better regulation effect than HR group. A total of 16 differential compounds were found in the aqueous extracts of HR and HPHR, of which 10 compounds in HPHR were up regulated, while 6 compounds were down regulated compare to HR.
The results indicated that both HR and HPHR can improve spleen qi deficiency syndrome of rats, the pharmacodynamic effect of the latter was better than the former. Differences in components of HR and HPHR potentially leading to variations in efficacy.
比较蜜炙前后红芪化学成分的变化，探讨红芪与蜜炙红芪在补中益气方面差异的物质基础。
采用超高效液相色谱-质谱联用技术分析红芪及蜜炙红芪水提取物中的不同化合物。建立大鼠脾气虚模型。将大鼠分别用不同剂量的红芪或蜜炙红芪水提取物进行处理，以脾组织病理差异、血清木糖、胃泌素（GAS）和淀粉酶（AMS）水平、白细胞介素-2（IL）-2和肿瘤坏死因子-α（TNF-α）以及脾和胸腺指数为指标，研究红芪与蜜炙红芪在补中益气方面的药效差异。
研究表明，与空白组相比，模型组大鼠脾组织出现损伤，主要表现为红髓与白髓界限不清、脾脏形态不规则、排列紊乱、白髓结构破坏、淋巴细胞减少、生发中心数量减少或萎缩。与模型组相比，红芪及蜜炙红芪中、高剂量组对脾气虚大鼠脾组织有保护作用，且蜜炙红芪作用更强；与模型组相比，各治疗组大鼠血清木糖、GAS和AMS水平及胸腺和脾脏指数显著升高，血清IL-2和TNF-α水平显著降低，其中蜜炙红芪组调节作用优于红芪组。红芪与蜜炙红芪水提取物中共发现16种差异化合物，其中蜜炙红芪中有10种化合物上调，6种化合物与红芪相比下调。
结果表明，红芪和蜜炙红芪均能改善大鼠脾气虚证，后者药效优于前者。红芪与蜜炙红芪成分差异可能导致药效不同。
