Yoon Dahye, Kim Ye Jin, Lee Wan Kyu, Choi Bo Ram, Oh Seon Min, Lee Young Seob, Kim Jae Kwang, Lee Dae Young
Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong 27709, Korea.
Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Yeonsugu, Incheon 22012, Korea.
Metabolites. 2020 Dec 19;10(12):517. doi: 10.3390/metabo10120517.
The effects of black ginseng, which has many kinds of biological activities, on dogs was investigated. Serum samples of beagle dogs, which were fed with black ginseng for 8 weeks, were measured using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectrometry. Acquired NMR data from the serum of dogs fed for 0, 4, and 8 weeks were analyzed by metabolic profiling and multivariate statistical analysis. In statistical analysis and biomarker analysis results of metabolite profiles, formate, glutamine, histidine, isoleucine, leucine, proline, and valine had variable importance in projection (VIP) scores above 1.0 and excellent area under the curve (AUC) values of receiver operating characteristic (ROC) curves above 0.9. In the result of multivariate statistical analysis, the score plot showed the discrimination between before and after feeding of black ginseng. These differences in metabolic profiles are considered to be due to the involvement of metabolic processes following black ginseng administration, such as enhancing immunity and energy metabolism. Through metabolomics analysis, we confirmed the biological efficacy of black ginseng in dogs and also confirmed that metabolomics can be applied to the pet health industry.
研究了具有多种生物活性的黑参对犬类的影响。对食用黑参8周的比格犬的血清样本，使用高分辨率魔角旋转（HR-MAS）核磁共振（NMR）光谱法进行测量。通过代谢谱分析和多变量统计分析，对喂食0周、4周和8周的犬血清所获得的NMR数据进行分析。在代谢物谱的统计分析和生物标志物分析结果中，甲酸、谷氨酰胺、组氨酸、异亮氨酸、亮氨酸、脯氨酸和缬氨酸的投影变量重要性（VIP）得分高于1.0，且受试者工作特征（ROC）曲线的曲线下面积（AUC）值优异，高于0.9。在多变量统计分析结果中，得分图显示了喂食黑参前后的差异。这些代谢谱的差异被认为是由于黑参给药后代谢过程的参与，如增强免疫力和能量代谢。通过代谢组学分析，我们证实了黑参对犬类的生物学功效，也证实了代谢组学可应用于宠物健康产业。
