Ahn Seunghyun, Lee Jung Yeon, Choi Sang Mi, Shin Yujeong, Park Seyeon
Department of Applied Chemistry, Dongduk Women's University, Seoul 02748, Korea.
Pharmaceutics. 2020 Dec 18;12(12):1234. doi: 10.3390/pharmaceutics12121234.
Oral finasteride and topical minoxidil are single components approved by the US FDA for treating hair loss. Some other compounds originating from natural products are also traditionally used for promoting hair growth. In this study, observations of treated keratinocyte cells were used to demonstrate that tocopherol acetate, L-menthol, and stevioside exert an effect on cell regeneration. Furthermore, these were topically applied to the shaved skin of C57BL/6 mice to observe their effects on hair growth. A mixture of tocopherol acetate, L-menthol, and stevioside showed the highest potential for promoting hair growth in vivo. In in vivo experiments, the mixture of tocopherol acetate, L-menthol, and stevioside was more effective than tocopherol acetate or L-menthol alone in promoting hair growth. The transcriptome analysis of skin from the dorsal side of a mouse treated with tocopherol acetate or L-menthol versus vehicle revealed key changes in keratin, keratin-associated protein, forkhead box, sonic hedgehog, fibroblast growth factor 10, desmoglein 4, deoxyribonuclease 1-like 2, and cadherin 3, known to play roles in promoting hair growth.
口服非那雄胺和外用米诺地尔是美国食品药品监督管理局（US FDA）批准用于治疗脱发的单一成分。一些源自天然产物的其他化合物传统上也用于促进头发生长。在本研究中，通过对经处理的角质形成细胞的观察来证明醋酸生育酚、L-薄荷醇和甜菊糖苷对细胞再生有影响。此外，将这些物质局部应用于C57BL/6小鼠剃毛的皮肤，以观察它们对毛发生长的影响。醋酸生育酚、L-薄荷醇和甜菊糖苷的混合物在体内显示出促进毛发生长的最大潜力。在体内实验中，醋酸生育酚、L-薄荷醇和甜菊糖苷的混合物在促进毛发生长方面比单独使用醋酸生育酚或L-薄荷醇更有效。对用醋酸生育酚或L-薄荷醇处理的小鼠背部皮肤与赋形剂进行转录组分析，揭示了角蛋白、角蛋白相关蛋白、叉头框、音猬因子、成纤维细胞生长因子10、桥粒芯糖蛋白4、脱氧核糖核酸酶1样2和钙黏蛋白3中的关键变化，这些已知在促进毛发生长中起作用。
