Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center, Rochester, NY 14642, USA; Department of Neurosurgery, the Fourth Affiliated Hospital of China Medical University, Shenyang 110032, China.
Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center, Rochester, NY 14642, USA; Department of Neuroscience, University of Rochester Medical Center, Rochester, NY 14642, USA.
Cell Rep. 2020 Dec 22;33(12):108524. doi: 10.1016/j.celrep.2020.108524.
The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4 ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-β. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked.
对脑液转运的新兴兴趣促使人们需要一些技术来了解哪些因素调节脑脊液（CSF）的产生。在这里，我们描述了一种在清醒小鼠中直接定量 CSF 产生的方法。我们通过将导管放置在侧脑室中来测量 CSF 的产生，同时物理阻断来自第四脑室的流出。使用这种方法，我们发现与清醒状态相比，异氟烷麻醉期间 CSF 的产生增加，而在氯胺酮/甲苯噻嗪麻醉期间增加的程度较小。年老小鼠的 CSF 产生减少，而过表达淀粉样蛋白-β的老年小鼠的 CSF 产生甚至更低。出乎意料的是，年轻雌性小鼠的 CSF 产生比同龄雄性小鼠高 30%。总之，目前的观察结果表明，CSF 产生的减少可能导致与年龄相关的蛋白病风险，但 CSF 产生的速度和糖液转运之间没有直接联系。
