Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India.
Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India.
Virus Res. 2021 Mar;294:198275. doi: 10.1016/j.virusres.2020.198275. Epub 2021 Jan 8.
Tmprss2 is an emerging molecular target which guides cellular infections of SARS-CoV-2, has been earmarked for interventions against the viral pathologies. The study aims to computationally screen and identifies potential miRNAs, following in vitro experimental validation of miRNA-mediated suppression of Tmprss2 for early prevention of COVID-19. Pool of 163 miRNAs, scrutinized for Tmprss2 binding with three miRNA prediction algorithms, ensued 11 common miRNAs. Further, computational negative energies for association, corroborated miRNA-Tmprss2 interactions, whereas three miRNAs (hsa-miR-214, hsa-miR-98 and hsa-miR-32) based on probability scores ≥0.8 and accessibility to Tmprss2 target have been selected in the Sfold tool. Transfection of miRNA(s) in the Caco-2 cells, quantitatively estimated differential expression, confirming silencing of Tmprss2 with maximum gene suppression by hsa-miR-32 employing novel promising role in CoV-2 pathogenesis. The exalted binding of miRNAs to Tmprss2 and suppression of later advocates their utility as molecular tools for prevention of SARS-CoV-2 viral transmission and replication in humans.
Tmprss2 是一种新兴的分子靶点，指导 SARS-CoV-2 的细胞感染，已被指定用于干预病毒病理学。本研究旨在通过体外实验验证 miRNA 介导的 Tmprss2 抑制作用，以进行 COVID-19 的早期预防，从而进行计算筛选和鉴定潜在的 miRNA。对 163 种 miRNA 进行了筛选，用三种 miRNA 预测算法对 Tmprss2 进行了分析，得到了 11 种常见的 miRNA。进一步，计算出的结合能证实了 miRNA-Tmprss2 的相互作用，而基于概率评分≥0.8 和对 Tmprss2 靶标可达性的三种 miRNA（hsa-miR-214、hsa-miR-98 和 hsa-miR-32）已被选中在 Sfold 工具中进行研究。在 Caco-2 细胞中转染 miRNA(s)，定量估计差异表达，证实了 hsa-miR-32 对 Tmprss2 的沉默作用，这表明其在 CoV-2 发病机制中具有新的潜在作用。miRNA 与 Tmprss2 的高度结合以及对后者的抑制作用表明，它们可用作预防 SARS-CoV-2 病毒在人类中传播和复制的分子工具。
