（脂鲤目：半线脂鲤属）的完整线粒体基因组及脂鲤目的系统发育研究
The complete mitochondrial genome of (Characiformes: Hemigrammus) and phylogenetic studies of Characiformes.
作者信息
Wang Qi, Miao Zengliang, Chen Jian, Huang Youkun, Meng Fang, Zhu Kehua, Liu Bingjian, Liu Yifan
机构信息
National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, Zhejiang, China.
National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, Zhejiang, China.
出版信息
Mitochondrial DNA B Resour. 2019 Nov 5;4(2):3834-3835. doi: 10.1080/23802359.2019.1681309.
Complete mitochondrial genome of the characiform fish was characterized in the present study. The whole mitogenome was 17,021 bp in size and consisted of 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs genes, a control region, and origin of light-strand replication. The proportion of coding sequences with a total length of 11,415 bp is 67.06%, which encodes 3805 amino acids. Similar to other species, the base composition of was 29.30% for A, 25.26% for C, 16.36% for G, and 29.08% for T. All PCGs started with Met. , , , , and ended with TAA as the stop codon. , , and ended with TAG as a stop codon, , , , and ended simply by T, and ended by a single AGG. The lengths of 12S ribosomal RNA and 16S ribosomal RNA were 924 bp and 1681 bp, respectively. The length of control region (D-loop) was 1308 bp, ranging from 15,714 to 17,021 bp. The complete mitochondrial genome sequence provided here would be helpful in further understanding the evolution of characiformes and conservation genetics of .
本研究对脂鲤形目鱼类的完整线粒体基因组进行了表征。整个线粒体基因组大小为17,021 bp，由13个蛋白质编码基因（PCGs）、22个tRNA、2个rRNA基因、一个控制区和轻链复制起点组成。编码序列总长度为11,415 bp，占比67.06%，编码3805个氨基酸。与其他物种相似，其碱基组成为：A占29.30%，C占25.26%，G占16.36%，T占29.08%。所有PCGs均以Met起始。 、 、 、 和 以TAA作为终止密码子结束。 、 和 以TAG作为终止密码子结束， 、 、 、 和 仅以T结束， 以单个AGG结束。12S核糖体RNA和16S核糖体RNA的长度分别为924 bp和1681 bp。控制区（D-loop）长度为1308 bp，范围从15,714至17,021 bp。此处提供的完整线粒体基因组序列将有助于进一步了解脂鲤形目的进化以及 的保护遗传学。
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