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鲤齿目的完整线粒体基因组及系统发育研究

The complete mitochondrial genome of and phylogenetic studies of Cyprinodontidaes.

作者信息

Ren Chuanchen, Zhao Xinyu, Meng Fang, Liu Yifan, Wang Hongqiang, Zhang Kun, Liu Bingjian, Yang Enshang

机构信息

National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China.

National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, China.

出版信息

Mitochondrial DNA B Resour. 2021 Aug 31;6(10):2796-2798. doi: 10.1080/23802359.2021.1970633. eCollection 2021.

Abstract

The complete mitochondrial genome of the Cyprinodontiformes fish was studied in this study. The size of the entire mitochondrial genome was 16,878 bp, and the total length of the encoding sequence was 11,435 bp, accounting for 67.75%, encoding 3800 amino acids in total. Genome base compositions were: A was 27.30%, C was 28.30%, G was 16.17%, and T was 28.23%. ND2 and CO1 started with GTG, and other protein-coding genes started with ATG. ND1, ND2, and ND3 ended with TAG, respectively, CO2 and ND4 ended with a single T, and other PCGS ended with TAA. The ribosomal RNA lengths of 12S and 16S were 950 bp and 1690 bp, respectively. The control area (D-loop) was 1194 bp in size and ranged from 15,685 to 16,878 bp. It showed negative GC skew value (-0.2728) and negative AT skewness (-0.0168). Phylogenetic analysis showed that was most closely related to . The complete sequence of the mitochondrial genome will provide a new perspective for classification and help to draw a more complete picture of species diversity within the Cyprinodontiformes.

摘要

本研究对鲤齿目鱼类的完整线粒体基因组进行了研究。整个线粒体基因组大小为16,878 bp,编码序列总长度为11,435 bp,占67.75%,共编码3800个氨基酸。基因组碱基组成为:A占27.30%,C占28.30%,G占16.17%,T占28.23%。ND2和CO1以GTG起始,其他蛋白质编码基因以ATG起始。ND1、ND2和ND3分别以TAG结尾,CO2和ND4以单个T结尾,其他PCGS以TAA结尾。12S和16S核糖体RNA长度分别为950 bp和1690 bp。控制区(D-loop)大小为1194 bp,范围在15,685至16,878 bp之间。其GC偏斜值为负(-0.2728),AT偏斜度为负(-0.0168)。系统发育分析表明,[此处原文缺失相关信息]与[此处原文缺失相关信息]关系最为密切。线粒体基因组的完整序列将为分类提供新的视角,并有助于更全面地描绘鲤齿目内的物种多样性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/8425730/e8554c5f3c57/TMDN_A_1970633_F0001_B.jpg

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