（脂鲤目：线虫脂鲤属）的完整线粒体基因组及脂鲤科的系统发育研究
The complete mitochondrial genome of (Characiformes:Nematobrycon) and phylogenetic studies of Characidaes.
作者信息
Wang Qi, Zhang Tao, Yin Xiaolong, Meng Fang, Huang Youkun, Liu Bingjian, Liu Yifan
机构信息
National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China.
National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, China.
出版信息
Mitochondrial DNA B Resour. 2020 Oct 5;5(3):3474-3475. doi: 10.1080/23802359.2020.1825130.
Complete mitochondrial genome of the Characiform fish was characterized in the present study. The whole mitogenome was 17,340 bp in size and the proportion of coding sequences with a total length of 11,448 bp was 66.02%, which encodes 3805 amino acids. The base composition of the genome was 30.92% for A, 23.92% for C, 14.88% for G, and 30.28% for T. All protein-coding genes were started with ATG, CO1 and ATP8 ended by AGG, TAG respectively, whereas CO2, ATP6, ND4 ended by a single T, the other PCGs commonly ended by TAA. The length of 12S and 16S ribosomal RNA was 949 bp and 1675 bp, respectively. The control region (D-loop) ranging from 15,654 bp to 17,340 bp was 1687 bp in size. It showed negative GC skew value (-0.2329) and positive AT skewness (0.0105). Phylogenetic analysis showed that was most closely related to . The complete mitochondrial genome sequence would provide a new insight into taxonomic classification, and help to draw a more complete picture of species diversity within the Characidae.
本研究对脂鲤形目鱼类的完整线粒体基因组进行了表征。整个线粒体基因组大小为17,340 bp，编码序列全长11,448 bp，占比66.02%，编码3805个氨基酸。基因组的碱基组成为：A占30.92%，C占23.92%，G占14.88%，T占30.28%。所有蛋白质编码基因均以ATG起始，CO1和ATP8分别以AGG、TAG结束，而CO2、ATP6、ND4以单个T结束，其他蛋白质编码基因通常以TAA结束。12S和16S核糖体RNA的长度分别为949 bp和1675 bp。控制区（D-loop）范围为15,654 bp至17,340 bp，大小为1687 bp。其GC偏斜值为负（-0.2329），AT偏斜度为正（0.0105）。系统发育分析表明， 与 关系最为密切。完整的线粒体基因组序列将为分类学分类提供新的见解，并有助于更全面地描绘脂鲤科内的物种多样性。
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