（鲤齿目，鲤齿科）线粒体全基因组的系统发育分析
Phylogenetic analysis of the complete mitochondrial genome of (Cyprinodontiformes, Cyprinodontidae).
作者信息
Meng Fang, Chen Jian, Miao Zengliang, Zhu Kehua, Huang Youkun, Wang Qi, Liu Bingjian, Liu Yifan
机构信息
National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, China.
National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, China.
出版信息
Mitochondrial DNA B Resour. 2019 Nov 11;4(2):3884-3885. doi: 10.1080/23802359.2019.1687033.
The complete mitochondrial genome of this species was first determined in this study, which is 16,617 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, a putative control region, and 1 origin of replication on the light-strand. The overall base composition includes C(27.11%), A(26.68%), T(29.15%), G(17.04%) and three degenerate bases are R, R and S. Moreover, the 13 PCGs encode 3800 amino acids in total, 12 of which use the initiation codon ATG except COI that uses GTG. Most of them have TAA as the stop codon, whereas ND5 ends with AGA, four protein-coding genes (ND1, ND2, ND3 and Cytb) ended with TAG, and two protein-coding genes (COII and ND4) ended with the incomplete stop codon represented as a single T. The phylogenetic tree based on the Neighbor Joining method was constructed to provide relationship within Cyprinodontidae, which could be a useful basis for management of this species.
本研究首次测定了该物种的完整线粒体基因组，其长度为16,617 bp，包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因、1个假定的控制区和轻链上的1个复制起点。总体碱基组成为C（27.11%）、A（26.68%）、T（29.15%）、G（17.04%），三个简并碱基为R、R和S。此外，13个蛋白质编码基因共编码3800个氨基酸，其中12个使用起始密码子ATG，除COI使用GTG外。它们大多数以TAA作为终止密码子，而ND5以AGA结尾，四个蛋白质编码基因（ND1、ND2、ND3和Cytb）以TAG结尾，两个蛋白质编码基因（COII和ND4）以单个T表示的不完整终止密码子结尾。基于邻接法构建了系统发育树，以提供鲤齿科内部的关系，这可能是该物种管理的有用基础。
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