Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, P.R. China.
Food Funct. 2021 Jan 21;12(2):747-760. doi: 10.1039/d0fo02677h. Epub 2020 Dec 23.
The purposes of this study were to explore the preventive and treatment effects of Hunan insect tea polyphenols (HITPs) on gastric injury in mice induced by HCl/ethanol and to investigate their molecular mechanisms of action. Both HITPs and ranitidine inhibited the formation and further deterioration of gastric mucosal lesions, reduced the secretion of gastric juice, and raised gastric juice pH compared to the control. The HITPs-H treated group had lower serum levels of motilin, substance P, and endothelin than the control group, but they had higher serum levels of vasoactive intestinal peptide and somatostatin. Mice treated with HITPs had lower serum levels of cytokines interleukin (IL)-6, IL-12, tumor necrosis factor-α (TNF-α), and interferon-γ than the control group. The activities of superoxide dismutase (SOD), nitric oxide, and glutathione peroxidase (GSH-Px) were higher in the gastric tissues of HITP-treated mice, but the malondialdehyde content was lower. Quantitative PCR analysis indicated that the mRNA expression of occludin, epidermal growth factor (EGF), EGF receptor (EGFR), vascular EGF (VEGF), inhibitor kappaB-α, cuprozinc-superoxide dismutase, manganese-superoxide dismutase, GSH-Px, neuronal nitric oxide synthase, and endothelial NOS increased significantly in the gastric tissues of HITP-treated mice. However, the activated B cell, inducible NOS, cyclooxygenase-2, TNF-α, IL-1 beta, and IL-6 mRNA expression levels in the HITPs group were lower than those in the control group. The protective effect of a high concentration (200 mg per kg bw) of HITPs on gastric injury induced by HCl/ethanol was stronger than that of a low concentration (100 mg per kg bw) of HITPs. High-performance liquid chromatography (HPLC) revealed that the HITPs contained cryptochlorogenic acid, (-)-epicatechin gallate, and isochlorogenic acid C. Taken together, our findings indicate that the HITPs played a role in the prevention of gastric damage. The antioxidant effect of the HITPs contributed to their potential value in the prevention and treatment of gastric injury. HITPs have broad prospects as biologically active substances for food development.
本研究旨在探讨湖南虫茶多酚（HITPs）对 HCl/乙醇诱导的小鼠胃损伤的预防和治疗作用，并探讨其作用机制。与对照组相比，HITPs 和雷尼替丁均能抑制胃黏膜损伤的形成和进一步恶化，减少胃酸分泌，提高胃液 pH 值。与对照组相比，HITPs-H 处理组血清胃动素、P 物质和内皮素水平较低，但血管活性肠肽和生长抑素水平较高。HITPs 处理组小鼠血清细胞因子白细胞介素（IL）-6、IL-12、肿瘤坏死因子-α（TNF-α）和干扰素-γ水平低于对照组。与对照组相比，HITPs 处理组小鼠胃组织中超氧化物歧化酶（SOD）、一氧化氮和谷胱甘肽过氧化物酶（GSH-Px）活性升高，丙二醛含量降低。定量 PCR 分析表明，HITPs 处理组小鼠胃组织中紧密连接蛋白、表皮生长因子（EGF）、EGF 受体（EGFR）、血管 EGF（VEGF）、抑制因子 kappaB-α、铜锌超氧化物歧化酶、锰超氧化物歧化酶、GSH-Px、神经元型一氧化氮合酶和内皮型一氧化氮合酶的 mRNA 表达显著增加。然而，HITPs 组活化 B 细胞、诱导型 NOS、环氧化酶-2、TNF-α、IL-1β和 IL-6 的 mRNA 表达水平低于对照组。HITPs 高浓度（200mg/kgbw）对 HCl/乙醇诱导的胃损伤的保护作用强于低浓度（100mg/kgbw）。高效液相色谱（HPLC）显示 HITPs 含有隐绿原酸、（-）-表儿茶素没食子酸酯和异绿原酸 C。综上所述，本研究结果表明 HITPs 对胃损伤具有预防作用。HITPs 的抗氧化作用可能与其预防和治疗胃损伤的潜在价值有关。HITPs 作为生物活性物质在食品开发方面具有广阔的前景。
