Parker R S
Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853.
Am J Clin Nutr. 1988 Jan;47(1):33-6. doi: 10.1093/ajcn/47.1.33.
Concentrations of individual carotenoids and tocopherols were determined in abdominal adipose tissue from 19 adults undergoing corrective surgery. Samples were extracted and saponified before separation and quantitation of carotenoids and tocopherols by reverse-phase high-performance liquid chromatography. Total carotenoid concentration varied 40-fold between individuals, from 0.34 to 13.51 micrograms/g adipose tissue. Beta-carotene and lycopene were the predominant carotenoids, averaging 20.2 and 18.5% of total carotenoids, respectively. In 10 of 19 subjects, the lycopene concentration exceeded that of beta-carotene. Total tocopherol concentrations varied 11-fold, with alpha-tocopherol representing 80.6 +/- 8.1% of the total. Absolute concentrations of both carotenoids and tocopherols were more variable than their relative concentrations. Both beta-carotene and lycopene concentrations were highly correlated with total carotenoid content but there was no correlation between beta-carotene and lycopene or between beta-carotene and alpha-tocopherol concentrations.
测定了19名接受矫正手术的成年人腹部脂肪组织中各胡萝卜素和生育酚的浓度。在通过反相高效液相色谱法分离和定量胡萝卜素及生育酚之前，先对样品进行提取和皂化处理。个体间总胡萝卜素浓度相差40倍，范围为0.34至13.51微克/克脂肪组织。β-胡萝卜素和番茄红素是主要的胡萝卜素，分别平均占总胡萝卜素的20.2%和18.5%。在19名受试者中的10名中，番茄红素浓度超过了β-胡萝卜素浓度。总生育酚浓度相差11倍，其中α-生育酚占总量的80.6±8.1%。胡萝卜素和生育酚的绝对浓度比它们的相对浓度变化更大。β-胡萝卜素和番茄红素浓度均与总胡萝卜素含量高度相关，但β-胡萝卜素与番茄红素之间或β-胡萝卜素与α-生育酚浓度之间均无相关性。
