Ali Aaliya, Garg Prakrati, Goyal Rohit, Kaur Gurjot, Li Xiangkai, Negi Poonam, Valis Martin, Kuca Kamil, Kulshrestha Saurabh
Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, Solan 173229, Himachal Pradesh, India.
Center for Omics and Biodiversity Research, Shoolini University of Biotechnology and Management Sciences, Bajhol, Solan 173229, Himachal Pradesh, India.
Plants (Basel). 2020 Dec 24;10(1):25. doi: 10.3390/plants10010025.
Treatment of wounds is essential as the wound can also be lethal at some point in time if not healed properly. Ethnomedicinal plants can treat wounds as they have no side effects, whereas, in the case of chemical drugs, the side effects are on the rise. In this study, seeds of which is the essential ethnomedicinal plant, were studied for wound healing efficacy. The study was planned for the assessment of in vitro (antioxidant and antimicrobial activities) and in vivo (excision and incision wound healing models) wound healing efficacy of n-hexane extract and hydrogels of seeds. The antioxidant and antimicrobial activities were assessed by DPPH free radical scavenging assay and Agar well diffusion method, respectively. In excision and incision wound models, Swiss albino mice were used for wound healing efficacy of hydrogels, i.e., 5% and 10% hexane extracts of seeds. The n-hexane extract showed antioxidant as well as antibacterial activities. Moreover, the hydrogels formulated using n-hexane extract of seeds showed significant wound healing activity compared to both control and standard until the end of the protocol in both the models. Furthermore, the histopathological investigation confirmed the findings of accelerated regeneration of tissue accompanied by a decrease in inflammatory cells and increased vascularity of the immediate skin. The results (both in vitro and in vivo) claimed conclusively that our n-hexane hydrogel formulation of seeds might serve as an alternative therapy in skin restoration during wound healing.
伤口治疗至关重要，因为如果伤口没有得到妥善愈合，在某些时候可能会致命。民族药用植物可以治疗伤口，因为它们没有副作用，而化学药物的副作用却在不断增加。在这项研究中，对作为重要民族药用植物的[植物名称]种子的伤口愈合功效进行了研究。该研究旨在评估[植物名称]种子正己烷提取物和水凝胶的体外（抗氧化和抗菌活性）以及体内（切除和切口伤口愈合模型）伤口愈合功效。抗氧化和抗菌活性分别通过DPPH自由基清除试验和琼脂扩散法进行评估。在切除和切口伤口模型中，使用瑞士白化小鼠来评估水凝胶（即5%和10%[植物名称]种子正己烷提取物）的伤口愈合功效。正己烷提取物显示出抗氧化以及抗菌活性。此外，与对照组和标准组相比，使用[植物名称]种子正己烷提取物配制的水凝胶在两个模型的实验方案结束前均显示出显著的伤口愈合活性。此外，组织病理学研究证实了组织加速再生的结果，同时炎症细胞减少，紧邻皮肤的血管增多。（体外和体内）结果最终表明，我们的[植物名称]种子正己烷水凝胶制剂可能作为伤口愈合过程中皮肤修复的替代疗法。
