Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Nutrients. 2020 Dec 25;13(1):42. doi: 10.3390/nu13010042.
Dietary fiber intake during pregnancy may improve offspring intestinal development. The aim of this study was to evaluate the effect of maternal high fiber intake during late gestation on intestinal morphology, microbiota, and intestinal proteome of newborn piglets. Sixteen sows were randomly allocated into two groups receiving the control diet (CD) and high-fiber diet (HFD) from day 90 of gestation to farrowing. Newborn piglets were selected from each litter, named as CON and Fiber group, respectively. Maternal high fiber intake did not markedly improve the birth weight, but increased the body length, the ileal crypt depth and colonic acetate level. In addition, maternal high fiber intake increased the -diversity indices (Observed species, Simpson, and ACE), and the abundance of and at phylum level, significantly increased the abundance of and at genus level in the colon of newborn piglets. Moreover, maternal high fiber intake markedly altered the ileal proteome, increasing the abundances of proteins associated with oxidative status, energy metabolism, and immune and inflammatory responses, and decreasing abundances of proteins related to cellular apoptosis, cell structure, and motility. These findings indicated that maternal high fiber intake could alter intestinal morphology, along with the altered intestinal microbiota composition and proteome of offspring.
孕期膳食纤维摄入可改善后代肠道发育。本研究旨在评估妊娠晚期母体高纤维摄入对新生仔猪肠道形态、微生物群和肠道蛋白质组的影响。16 头母猪随机分为两组，从妊娠第 90 天到分娩分别接受对照饮食（CD）和高纤维饮食（HFD）。从每个窝中选择新生仔猪，分别命名为 CON 和 Fiber 组。母体高纤维摄入并未显著提高出生体重，但增加了体长、回肠隐窝深度和结肠乙酸盐水平。此外，母体高纤维摄入增加了 -多样性指数（Observed species、Simpson 和 ACE），并增加了新生仔猪结肠中门水平的 和 的丰度，显著增加了属水平上的 和 的丰度。此外，母体高纤维摄入显著改变了回肠蛋白质组，增加了与氧化状态、能量代谢以及免疫和炎症反应相关的蛋白质的丰度，并降低了与细胞凋亡、细胞结构和运动性相关的蛋白质的丰度。这些发现表明，母体高纤维摄入可以改变后代的肠道形态，以及肠道微生物群组成和蛋白质组。
