AuBuchon J P, Estep T N, Davey R J
Department of Transfusion Medicine, National Institutes of Health, Bethesda, MD.
Blood. 1988 Feb;71(2):448-52.
Recent in vitro studies have shown that di-2-ethylhexyl-phthalate (DEHP) inhibits the deterioration of RBCs during refrigerated storage in containers that use this compound as a plasticizer. The experiments described in this report were designed to assess whether this in vitro protective effect of DEHP would result in a prolonged in vivo survival of RBCs infused into normal human recipients. Whole blood collected from ten normal donors was stored for 35 days in citrate-phosphate-dextrose-adenine (CPDA-1) anticoagulant contained in polyvinylchloride (PVC) bags plasticized with DEHP or a trimellitate compound that is known to have low leachability. Aliquots of RBCs from each container were then labeled with chromium-51 and were reinfused into the original donors. For blood stored in DEHP-plasticized PVC bags, 24% more red cells survived in vivo 24 hours after reinfusion than was observed when the blood had been stored in trimellitate-plasticized bags (P less than .001). Whole blood stored in glass bottles showed a similar improvement in in vivo survival when DEHP was added in weekly increments to mimic the accumulation of this plasticizer seen during storage in plastic containers. Survival of packed red cells stored in the presence of DEHP increased by 14% compared with storage in trimellitate-plasticized bags (P less than .05). In agreement with previous studies, hemolysis and microvesicle formation were also reduced in the presence of DEHP. These results suggest that proposed new storage systems lacking DEHP should be carefully evaluated to determine whether adequate post-transfusion survival of RBCs may be achieved.
最近的体外研究表明，邻苯二甲酸二（2-乙基己基）酯（DEHP）可抑制红细胞在使用该化合物作为增塑剂的容器中冷藏储存期间的变质。本报告中描述的实验旨在评估DEHP的这种体外保护作用是否会导致输注到正常人类受者体内的红细胞在体内存活时间延长。从10名正常供体采集的全血在含有DEHP或已知浸出率低的偏苯三酸酯化合物增塑的聚氯乙烯（PVC）袋中的柠檬酸-磷酸盐-葡萄糖-腺嘌呤（CPDA-1）抗凝剂中储存35天。然后将每个容器中的红细胞等分试样用铬-51标记，并重新输注到原始供体中。对于储存在DEHP增塑的PVC袋中的血液，再输注后24小时体内存活的红细胞比储存在偏苯三酸酯增塑袋中的血液多24%（P小于0.001）。当每周递增添加DEHP以模拟在塑料容器中储存期间所见的这种增塑剂的积累时，储存在玻璃瓶中的全血在体内存活方面也有类似改善。与储存在偏苯三酸酯增塑袋中相比，在DEHP存在下储存的浓缩红细胞的存活率提高了14%（P小于0.05）。与先前的研究一致，在DEHP存在下溶血和微泡形成也减少。这些结果表明，应仔细评估缺乏DEHP的拟议新储存系统，以确定是否可以实现红细胞充分的输血后存活。
