Shanker S, Lee A, Sorrell T C
Bacteriology Department, Institute of Clinical Pathology and Medical Research, Westmead Hospital, N.S.W., Australia.
Epidemiol Infect. 1988 Feb;100(1):27-34. doi: 10.1017/s0950268800065523.
Minimal colonization inocula for two broiler strains of Campylobacter jejuni were determined in broiler chicks aged 2-3 days and 2 weeks. Individually housed chicks were exposed to a single oral or cloacal challenge. Diarrhoeal symptoms were absent in all 380 chicks included in the study. Chick susceptibility to the two C. jejuni strains varied. Colonization was effected by less than 10(2)-10(4) colony forming units (c.f.u.) via cloacal challenge and 10(4)-10(6) c.f.u. via the oral route. Colonization inocula for 2- to 3-day and 2-week-old chicks were similar. Treatment of 1-day-old chicks with fresh adult caecal flora or an anaerobic broth culture of adult caecal flora did not inhibit colonization after challenge with low-dose C. jejuni. Susceptible chicks were colonized rapidly. C. jejuni was detected in 167 of 189 (88%) colonized chicks within 3 days of challenge and persisted during the 2-week monitoring period. Our data suggest that colonization of broiler chicks with C. jejuni is effected more easily by the cloacal than the oral route and is independent of age.
在2至3日龄和2周龄的肉鸡雏鸡中测定了空肠弯曲菌两个肉鸡菌株的最小定殖接种量。单独饲养的雏鸡接受单次口服或泄殖腔接种。该研究纳入的380只雏鸡均未出现腹泻症状。雏鸡对两种空肠弯曲菌菌株的易感性有所不同。通过泄殖腔接种，少于10(2)-10(4)个菌落形成单位（cfu）即可实现定殖，而通过口服途径则需要10(4)-10(6) cfu。2至3日龄和2周龄雏鸡的定殖接种量相似。用新鲜的成年盲肠菌群或成年盲肠菌群的厌氧肉汤培养物处理1日龄雏鸡，在低剂量空肠弯曲菌接种后并未抑制定殖。易感雏鸡迅速被定殖。在接种后3天内，189只定殖雏鸡中有167只（88%）检测到空肠弯曲菌，并且在2周的监测期内持续存在。我们的数据表明，空肠弯曲菌对肉鸡雏鸡的定殖通过泄殖腔途径比口服途径更容易实现，且与年龄无关。
