Stern N J, Bailey J S, Blankenship L C, Cox N A, McHan F
Russell Agricultural Research Center, USDA--Agricultural Research Service, Athens, Georgia 30613.
Avian Dis. 1988 Apr-Jun;32(2):330-4.
We report our findings on several parameters influencing cecal colonization of chickens by Campylobacter jejuni. Thirty-five colony-forming units (CFU) of a composite culture of C. jejuni colonized the ceca of one-half of the newly hatched chicks challenged by oral gavage. A challenge dose of 3500 CFU/chick consistently colonized the ceca of all chicks challenged. Challenge doses of approximately 10(5) CFU of C. jejuni per chick resulted in consistent cecal colonization, regardless of whether the birds were challenged 1, 2, or 3 days post-hatch. Four isolates showed consistently strong cecal colonization abilities, whereas two isolates colonized the ceca in only 20 of 122 chicks when given levels of 10(5) CFU per chick. One of these poorly colonizing isolates was repeatedly transferred by fecal-oral passage through chicks; subsequently, this isolate was able to consistently colonize chicks. Competitive exclusion (CE) microflora did not diminish the colonization rates for C. jejuni. Birds treated with five different CE cultures were colonized at a rate of 81 of 84 chicks; control chicks were similarly consistently colonized (45 of 46 chicks).
我们报告了关于影响空肠弯曲菌在鸡盲肠定植的几个参数的研究结果。通过口服灌胃对新孵化的雏鸡进行挑战，空肠弯曲菌复合培养物的35个菌落形成单位（CFU）定植于一半雏鸡的盲肠。每只雏鸡3500 CFU的挑战剂量始终能使所有受挑战雏鸡的盲肠定植。每只雏鸡约10⁵ CFU的空肠弯曲菌挑战剂量导致盲肠持续定植，无论雏鸡在孵化后1天、2天还是3天受到挑战。四个分离株表现出始终很强的盲肠定植能力，而当每只雏鸡给予10⁵ CFU水平时，两个分离株仅在122只雏鸡中的20只雏鸡的盲肠中定植。这些定植能力差的分离株之一通过经雏鸡粪口途径反复传代；随后，该分离株能够始终定植雏鸡。竞争性排斥（CE）微生物群并未降低空肠弯曲菌的定植率。用五种不同CE培养物处理的雏鸡，84只中有81只定植；对照雏鸡同样始终定植（46只中有45只）。
