Department of Bioengineering, Rice University, 6100 Main St. Houston, TX 77030, United States of America.
Department of Bioengineering, Rice University, 6100 Main St. Houston, TX 77030, United States of America.
J Control Release. 2021 Feb 10;330:565-574. doi: 10.1016/j.jconrel.2020.12.055. Epub 2020 Dec 31.
Mesenchymal stem cells (MSCs) are used extensively in developing tissue engineered constructs for bone and cartilage regeneration. An important factor in designing such constructs is that the MSCs are appropriately primed to differentiate along osteogenic or chondrogenic lineage. In contrast to a top-down method of tissue engineering where the differentiation of cells is guided by the scaffold and signals, a bottom-up method involves direct modulation of stem cell behavior without relying on the environmental cues. In this review, we discuss several bottom-up strategies that have emerged in engineering MSC behavior for bone and cartilage tissue engineering, including gene delivery, gene editing, and subpopulation isolation.
间充质干细胞（MSCs）广泛应用于开发用于骨和软骨再生的组织工程构建体。在设计此类构建体时，一个重要因素是适当诱导 MSCs 沿着成骨或成软骨谱系分化。与通过支架和信号引导细胞分化的自上而下的组织工程方法相反，自下而上的方法涉及在不依赖环境线索的情况下直接调节干细胞行为。在这篇综述中，我们讨论了几种用于骨和软骨组织工程的 MSC 行为工程的自下而上策略，包括基因传递、基因编辑和亚群分离。
