Department of Marine Biology, College of Oceanography, Hohai University, Nanjing, 210098, China.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Innovation Academy of Seed Design, Chinese Academy of Sciences, Wuhan, 430072, China.
Microb Biotechnol. 2022 Feb;15(2):683-702. doi: 10.1111/1751-7915.13741. Epub 2021 Jan 4.
Environmental bacteria have a great impact on fish gut microbiota, yet little is known as to where fish acquire their gut symbionts, and how gut microbiota response to the disturbance from environmental bacteria. Through the integrative analysis by community profiling and source tracking, we show that feed-associated bacteria can impose a strong disturbance upon the hindgut microbiota of cultured fugu. Consequently, marked alterations in the composition and function of gut microbiota in slow growth fugu were observed, implying a reduced stability upon bacterial disturbance from feed. Moreover, quantitative ecological analyses indicated that homogeneous selection and dispersal limitation largely contribute to the community stability and partial variations among hosts in the context of lower degree of disturbance. While the disturbance peaked, variable selection leads to an augmented interaction within gut microbiota, entailing community unstability and shift. Our findings emphasized the intricate linkage between feed and gut microbiota and highlighted the importance of resolving the feed source signal before the conclusion of comparative analysis of microbiota can be drawn. Our results provide a deeper insight into aquaculture of fugu and other economically important fishes and have further implications for an improved understanding of host-microbe interactions in the vertebrate gastrointestinal tract.
环境细菌对鱼类肠道微生物群有很大的影响，但对于鱼类从何处获得肠道共生体以及肠道微生物群如何对环境细菌的干扰做出反应，人们知之甚少。通过群落分析和来源追踪的综合分析，我们表明，饲料相关细菌会对养殖河豚的后肠微生物群造成强烈干扰。因此，在生长缓慢的河豚中观察到肠道微生物群的组成和功能发生了明显的改变，这意味着在受到饲料细菌干扰时，稳定性降低。此外，定量生态分析表明，在较低干扰程度的情况下，同质选择和扩散限制在很大程度上有助于群落的稳定性和宿主之间的部分变异。当干扰达到峰值时，可变选择导致肠道微生物群内的相互作用增强，从而导致群落不稳定和转移。我们的研究结果强调了饲料和肠道微生物群之间的复杂联系，并强调了在得出关于微生物群的比较分析结论之前，必须解决饲料来源信号的重要性。我们的研究结果为河豚和其他经济重要鱼类的水产养殖提供了更深入的了解，并进一步有助于更好地理解脊椎动物胃肠道中的宿主-微生物相互作用。
