Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas 88710, México.
National Center for Technology Management (An agency of the Federal Ministry of Science and Technology (FMST), Nigeria, Obafemi Awolowo University, Ile-Ife).
Afr Health Sci. 2020 Mar;20(1):168-181. doi: 10.4314/ahs.v20i1.22.
species are multi-resistant bacteria with ability to cause opportunistic infections.
We isolated 45 species from soil, sewage and the clinic with the aim of investigating their susceptibility to commonly used antimicrobial agents.
The identities of isolates were confirmed with 16S rRNA gene sequence and MALDI-TOF analysis. Anti-microbial resistance, biofilm production and clonal diversity were also evaluated. The minimum inhibitory concentration technique as described by Clinical & Laboratory Standards Institute: CLSI Guidelines (CLSI) was employed for the evaluation of isolate susceptibility to antibiotics.
Forty-five species which include 36 environmental strains and 9 clinical strains of were considered in this study. 32 (88.9 %) environmental strains were identified to be , 2 (5.6 %) were , and 2 (5.6 %) cluster as spp. isolates were resistant to at least six of the antibiotics tested, including / ().
Environmental isolates from this study were resistant to SXT which is commonly used for the treatment of infections. This informs the need for good public hygiene as the environment could be a reservoir of multi-resistant bacteria. It also buttresses the importance of surveillance study in the management of bacterial resistance.
是一种具有引起机会性感染能力的多耐药细菌。
我们从土壤、污水和临床中分离出 45 株 ，旨在研究它们对常用抗菌药物的敏感性。
通过 16S rRNA 基因序列和 MALDI-TOF 分析确认分离株的身份。还评估了抗微生物耐药性、生物膜形成和克隆多样性。采用临床和实验室标准协会（CLSI）：CLSI 指南（CLSI）描述的最低抑菌浓度技术评估抗生素对分离株的敏感性。
在这项研究中考虑了 45 株 ，包括 36 株环境株和 9 株临床株。32 株（88.9%）环境株被鉴定为 ，2 株（5.6%）为 ，2 株（5.6%）聚类为 spp. 分离株对至少六种测试的抗生素具有耐药性，包括 / （）。
本研究从环境中分离出的 对常用于治疗 感染的 SXT 具有耐药性。这表明需要良好的公共卫生，因为环境可能是多耐药菌的储库。它还支持在细菌耐药性管理中进行监测研究的重要性。
