与良性前列腺增生患者相比，前列腺癌患者中 IL-10+CD19+B 细胞的频率。
Frequency of IL-10+CD19+ B cells in patients with prostate cancer compared to patients with benign prostatic hyperplasia.
机构信息
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Pathology, Shahrekord University of Medical Sciences, Shahrekord, Iran.
出版信息
Afr Health Sci. 2020 Sep;20(3):1264-1272. doi: 10.4314/ahs.v20i3.31.
BACKGROUND
The function of the immune system in prostate cancer (PC) might promote carcinogenesis. PC is a common cancer in men. Regulatory B cells (Bregs) are a new subtype of B cells that have suppressive roles in the immune system. Interleukin-10 (IL-10) is a dominant mediator of immune suppression released by Bregs.
OBJECTIVE
The purpose of this research was to examine the frequency of CD19+IL10+ B cells and IL-10 mRNA expression in patients with PC compared to patients with benign prostatic hyperplasia (BPH).
METHODS
Forty paraffin tissue samples from patients with PC and 32 paraffin tissue samples from patients with BPH were entered in this study. The immunohistochemistry staining was used to evaluate the pattern expression of CD19 and IL-10 markers. IL-10 mRNA expression in fresh tissue was determined by real time-polymerase chain reaction (RT-PCR).
RESULTS
The frequency of CD19+IL-10+ B cells and IL-10 mRNA expression in PC patients were significantly higher than patients with BPH. Also, there was no meaningful relationship between the frequency of IL-10+CD19+ B cells and gleason scores in patients with PC.
CONCLUSIONS
Our findings suggested that frequency of IL-10+CD19+ B cells correlates with progressive stage of PC.
背景
免疫系统在前列腺癌（PC）中的功能可能促进癌发生。PC 是男性常见的癌症。调节性 B 细胞（Bregs）是 B 细胞的一个新亚型，在免疫系统中具有抑制作用。白细胞介素 10（IL-10）是 Bregs 释放的主要免疫抑制介质。
目的
本研究旨在检测与良性前列腺增生（BPH）患者相比，PC 患者中 CD19+IL10+B 细胞和 IL-10mRNA 表达的频率。
方法
本研究纳入了 40 例 PC 患者和 32 例 BPH 患者的石蜡组织样本。采用免疫组织化学染色法评估 CD19 和 IL-10 标志物的表达模式。通过实时聚合酶链反应（RT-PCR）测定新鲜组织中 IL-10mRNA 的表达。
结果
PC 患者中 CD19+IL10+B 细胞的频率和 IL-10mRNA 表达明显高于 BPH 患者。此外，PC 患者中 IL-10+CD19+B 细胞的频率与 Gleason 评分之间无明显关系。
结论
我们的研究结果表明，IL-10+CD19+B 细胞的频率与 PC 的进展阶段相关。
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