Saghaei Elham, Nasiri Boroujeni Shakiba, Safavi Parvin, Borjian Boroujeni Zeinab, Bijad Elham
Department of Physiology and Pharmacology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Evid Based Complement Alternat Med. 2020 Dec 21;2020:5725361. doi: 10.1155/2020/5725361. eCollection 2020.
In the present experimental study, male NMRI mice were exposed to chronic unpredictable mild stress (CUMS) paradigm for 35 days. Diosmetin (at doses of 10, 20, and 40 mg/kg. i.p.) or diosmetin solvent (normal saline + DMSO, 1 ml/kg; i.p.) was administered 30 min before stress induction. After 28 days, memory and cognitive performance were assessed by shuttle box and novel object recognition tests. Finally, antioxidant capacity (FRAP) and malondialdehyde (MDA) level of serum and brain, and serum corticosterone level were evaluated.
Behavioral tests showed that CUMS significantly reduced the secondary latency in passive avoidance memory test and diagnosis index in novel object recognition test compared to the control group ( < 0.001), whereas treatment with diosmetin (20 and 40 mg/kg) significantly improved memory performance in the two tests ( < 0.001). In addition, diosmetin (40 mg/kg) could pronouncedly suppress increase in serum corticosterone levels, reduction in antioxidant capacity, and production of excess MDA caused by CUMS compared to the control group ( < 0.01, < 0.001, and < 0.001, respectively).
Chronic stress can impair memory and cognition and treatment with diosmetin can partly improve this disorder in male mice by increasing the antioxidant capacity of brain tissue and serum and improving serum corticosterone levels.
在本实验研究中，雄性NMRI小鼠接受35天的慢性不可预测轻度应激（CUMS）范式。在应激诱导前30分钟给予香叶木素（剂量为10、20和40mg/kg，腹腔注射）或香叶木素溶剂（生理盐水+二甲基亚砜，1ml/kg；腹腔注射）。28天后，通过穿梭箱和新物体识别测试评估记忆和认知能力。最后，评估血清和大脑的抗氧化能力（FRAP）、丙二醛（MDA）水平以及血清皮质酮水平。
行为测试表明，与对照组相比，CUMS显著降低了被动回避记忆测试中的二次潜伏期和新物体识别测试中的诊断指数（<0.001），而香叶木素（20和40mg/kg）处理显著改善了这两项测试中的记忆表现（<0.001）。此外，与对照组相比，香叶木素（40mg/kg）可显著抑制CUMS引起的血清皮质酮水平升高、抗氧化能力降低以及过量MDA的产生（分别为<0.01、<0.001和<0.001）。
慢性应激会损害记忆和认知，香叶木素治疗可通过提高脑组织和血清的抗氧化能力以及改善血清皮质酮水平，部分改善雄性小鼠的这种紊乱。
