The Diversity, Resistance Profiles and Plasmid Content of spp. Recovered from Dairy Farms Located around Three Cities in Pakistan.

The rise of antimicrobial resistance (AMR) in bacterial pathogens such as (Kp) is a pressing public health and economic concern. The 'One-Health' framework recognizes that effective management of AMR requires surveillance in agricultural as well as clinical settings, particularly in low-resource regions such as Pakistan. Here, we use whole-genome sequencing to characterise 49 isolates of spp. (including 43 Kp) and 2 presumptive isolates recovered from dairy farms located near 3 cities in Pakistan-Quetta ( = 29), Faisalabad ( = 19), and Sargodha ( = 3). The 43 Kp isolates corresponded to 38 sequence types (STs), and 35 of these STs were only observed once. This high diversity indicates frequent admixture and limited clonal spread on local scales. Of the 49 spp. isolates, 41 (84%) did not contain any clinically relevant antimicrobial resistance genes (ARGs), and we did not detect any ARGs predicted to encode resistance to carbapenems or colistin. However, four Kp lineages contained multiple ARGs: ST11 ( = 2), ST1391-1LV ( = 1), ST995 ( = 1) and ST985 ( = 1). STs 11, 1391-1LV and 995 shared a core set of five ARGs, including , harboured on different AMR plasmids. ST985 carried a different set of 16 resistance genes, including . The two presumptive isolates also contained multiple ARGs. Finally, the four most common plasmids which did not harbour ARGs in our dataset were non-randomly distributed between regions, suggesting that local expansion of the plasmids occurs independently of the host bacterial lineage. Evidence regarding how dairy farms contribute to the emergence and spread of AMR in Pakistan is valuable for public authorities and organizations responsible for health, agriculture and the environment, as well as for industrial development.