BRGM, Orléans Cedex 245060, France.
PRC, INRAE, CNRS, Université de Tours, IFCE, Nouzilly 37380, France.
Hum Reprod. 2021 Mar 18;36(4):1032-1042. doi: 10.1093/humrep/deaa365.
Do plastic laboratory consumables and cell culture media used in ART contain bisphenols?
The majority of human embryo culture media assessed contained bisphenol S close to the nanomolar concentration range, while no release of bisphenols by plastic consumables was detected under routine conditions.
The deleterious effect of the endocrine disruptor bisphenol A (BPA) on female fertility raised concerns regarding ART outcome. BPA was detected neither in media nor in the majority of plastic consumables used in ART; however, it might have already been replaced by its structural analogs, including bisphenol S (BPS).
STUDY DESIGN, SIZE, DURATION: Seventeen plastic consumables and 18 cell culture and ART media were assessed for the presence of bisphenols.
PARTICIPANTS/MATERIALS, SETTING, METHODS: Ten different bisphenols (bisphenol A, S, AF, AP, B, C, E, F, P and Z) were measured using an isotopic dilution according to an on-line solid phase extraction/liquid chromatography/mass spectrometry method.
While the plastic consumables did not release bisphenols under routine conditions, 16 of the 18 cell culture and ART media assessed contained BPS. Six media exhibited BPS concentrations higher than 1 nM and reached up to 6.7 nM (1693 ng/l).
N/A.
LIMITATIONS, REASONS FOR CAUTION: Further studies are required to investigate a greater number of ART media to identify less potentially harmful ones, in terms of bisphenol content.
As BPS has already been reported to impair oocyte quality at nanomolar concentrations, its presence in ART media, at a similar concentration range, could contribute to a decrease in the ART success rate. Thus far, there has been no regulation of these compounds in the ART context.
STUDY FUNDING/COMPETING INTERESTS: This study was financially supported by the 'Centre-Val de Loire' Region (Bemol project, APR IR 2017), INRAE, BRGM, the French National Research Agency (project ANR-18-CE34-0011-01 MAMBO) and the BioMedicine Agency (Project 18AMP006 FertiPhenol). The authors declare that they have no conflict of interest that could be perceived as prejudicing the impartiality of the reported research.
ART 中使用的塑料实验室耗材和细胞培养基是否含有双酚？
评估的大多数人类胚胎培养基中均含有接近纳摩尔浓度范围的双酚 S，而在常规条件下未检测到塑料耗材释放双酚。
内分泌干扰物双酚 A (BPA) 对女性生育能力的有害影响引起了人们对 ART 结果的担忧。在培养基和 ART 中使用的大多数塑料耗材中均未检测到 BPA；然而，它可能已经被其结构类似物（包括双酚 S (BPS)）所取代。
研究设计、规模、持续时间：评估了 17 种塑料耗材和 18 种细胞培养和 ART 培养基中双酚的存在情况。
参与者/材料、设置、方法：使用同位素稀释法，根据在线固相萃取/液相色谱/质谱法，对十种不同的双酚（双酚 A、S、AF、AP、B、C、E、F、P 和 Z）进行了测量。
虽然在常规条件下塑料耗材不会释放双酚，但评估的 18 种细胞培养和 ART 培养基中有 16 种含有 BPS。六种培养基的 BPS 浓度高于 1 纳米，最高可达 6.7 纳米（1693 纳克/升）。
无。
局限性、谨慎的原因：需要进一步研究以评估更多数量的 ART 培养基，以确定在双酚含量方面潜在危害较小的培养基。
由于 BPS 已被报道在纳摩尔浓度下会损害卵母细胞质量，因此其在 ART 培养基中的存在，处于类似的浓度范围，可能会导致 ART 成功率下降。到目前为止，这些化合物在 ART 环境中还没有受到监管。
研究资金/竞争利益：本研究由“中央大区”（Bemol 项目，APR IR 2017）、INRAE、BRGM、法国国家研究署（项目 ANR-18-CE34-0011-01 MAMBO）和生物医学局（项目 18AMP006 FertiPhenol）资助。作者声明他们没有可能被视为损害所报告研究公正性的利益冲突。
