National Research Ogarev Mordovia State University, Bolshevistskaya Str. 68, 430005, Saransk, Russia.
FSRC "Crystallography and Photonics" RAS, Leninsky pr. 59, 119333, Moscow, Russia.
Colloids Surf B Biointerfaces. 2021 Mar;199:111548. doi: 10.1016/j.colsurfb.2020.111548. Epub 2020 Dec 25.
Magnetic hyperthermia (MH) is a perspective tool to treat the tumor while the magnetic material is delivered. The key problems in MH development is to ensure an effective local heating within cancer cell without overheating other cells. In order to do that one has to reach substantial local accumulation of magnetic nanoparticles (MNPs) and/or magnetically sensitive objects with advanced heat properties. Absorbing heat energy for destroying tumor cells can be generated only if there is sufficient amount of locally placed MNPs. In this work, we propose polyelectrolyte microcapsules modified with iron oxide nanoparticles as an approach to tie magnetic materials in high concentration locally. These microcapsules (about 3 microns in diameter) can be readily internalized by various cells. The human fibroblasts uptake of the microcapsules and cytotoxic effect upon the influence of alternating magnetic field (AMF) while magnetic capsules are inside the cells is under study in this work. The cytotoxicity of the magnetic microcapsules was compared with the cytotoxicity of the MNPs while free in the solution to evaluate the effect of bounding MNPs. A cytotoxic effect on cells was found in the case of preliminary incubation of fibroblasts with capsules while the AMF is applied. In the case of MNPs in an equivalent dose per mass of magnetic material, there was no cytotoxic effect noticed after the treatment with the field. It is noteworthy that during the treatment of cells with the AMF, the increase in temperature of the incubation medium was not registered. The morphological changes on fibroblasts were consistent with the data of the viability assessment. Thus, the synthesized capsules are shown as a means for local enhancement of magnetic hyperthermia in the treatment of tumor diseases.
磁热疗（MH）是一种有前途的治疗肿瘤的方法，同时可以输送磁性材料。在 MH 开发中，关键问题是确保在不使其他细胞过热的情况下，在癌细胞内实现有效的局部加热。为了实现这一点，必须实现磁性纳米粒子（MNPs）和/或具有先进热性能的磁性敏感物体的大量局部积累。只有当有足够数量的局部放置的 MNPs 时，才能产生用于破坏肿瘤细胞的热能。在这项工作中，我们提出了用氧化铁纳米粒子修饰的聚电解质微胶囊作为将磁性材料局部高浓度结合的方法。这些微胶囊（直径约 3 微米）可以很容易地被各种细胞内化。这项工作研究了在交变磁场（AMF）的影响下，磁性微胶囊在细胞内时，人成纤维细胞对微胶囊的摄取和细胞毒性作用。将磁性微胶囊的细胞毒性与游离在溶液中的 MNPs 的细胞毒性进行了比较，以评估结合 MNPs 的效果。在初步孵育成纤维细胞与微胶囊的情况下，当施加 AMF 时，发现了对细胞的细胞毒性作用。在具有相同磁材料质量剂量的 MNPs 的情况下，在磁场处理后没有观察到细胞毒性作用。值得注意的是，在使用 AMF 处理细胞的过程中，未检测到孵育介质温度的升高。成纤维细胞的形态变化与细胞活力评估数据一致。因此，合成的微胶囊被证明是局部增强磁热疗治疗肿瘤疾病的一种手段。
