Khaled Jamal M, Alyahya Sami A, Chenthis Kanisha C, Alharbi Naiyf S, Kadaikunnan Shine, Ramachandran G, Alanzi Khalid F, Rajivgandhi G, Vimala Rtv, Manoharan N
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
National Center for Biotechnology, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia.
Saudi J Biol Sci. 2021 Jan;28(1):302-309. doi: 10.1016/j.sjbs.2020.10.003. Epub 2020 Oct 15.
Urinary tract infections are second most important diseases worldwide due to the increased amount of antibiotic resistant microbes. Among the Gram negative bacteria, is the dominant biofilm producer in urinary tract infections next to . Biofilm is a process that produced self-matrix of more virulence pathogens on colloidal surfaces. Based on the above fact, this study was concentrated to inhibit the biofilm formation by various experiments. In the current study, the anti-biofilm effect of essential oils was recovered from the medicinal plant of and confirmed the available essential oils by liquid chromatography-mass spectroscopy analysis. The excellent anti-microbial activity and minimum biofilm inhibition concentration of the essential oils against was indicated at 200 µg/mL. The absence of viability and altered exopolysaccharide structure of treated cells were showed by biofilm metabolic assay and phenol-sulphuric acid method. The fluorescence differentiation of treated cells was showed with more damages by confocal laser scanning electron microscope. Further, more morphological changes of essential oils treated cells were differentiated from normal cells by scanning electron microscope. Altogether, the results were reported that the essential oils have anti-biofilm ability.
由于抗生素耐药微生物数量的增加，尿路感染是全球第二重要的疾病。在革兰氏阴性菌中，[此处原文缺失具体细菌名称]是仅次于[此处原文缺失具体细菌名称]的尿路感染中主要的生物膜产生菌。生物膜是一种在胶体表面产生更多毒力病原体自身基质的过程。基于上述事实，本研究集中于通过各种[此处原文缺失具体实验名称]实验来抑制[此处原文缺失具体细菌名称]生物膜的形成。在当前研究中，从[此处原文缺失具体药用植物名称]的药用植物中提取了精油的抗生物膜作用，并通过液相色谱 - 质谱分析确认了可用的精油。精油对[此处原文缺失具体细菌名称]的优异抗菌活性和最低生物膜抑制浓度在200μg/mL时表现出来。生物膜代谢测定和苯酚 - 硫酸法显示了处理细胞的活力丧失和胞外多糖结构的改变。共聚焦激光扫描电子显微镜显示[此处原文缺失具体细菌名称]处理细胞的荧光分化有更多损伤。此外，扫描电子显微镜将精油处理细胞的更多形态变化与正常细胞区分开来。总之，结果表明[此处原文缺失具体细菌名称]精油具有抗生物膜能力。
