Liu Yang, Chang Juan, Wang Ping, Yin Qing-Qiang, Huang Wei-Wei, Liu Chao-Qi, Bai Xian-Xiao, Zhu Qun, Gao Tian-Zeng, Zhou Pu
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 40002, China.
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
AMB Express. 2019 Sep 3;9(1):137. doi: 10.1186/s13568-019-0863-9.
Deoxynivalenol (DON) is one of the mycotoxins most frequently encountering in cereal-based foods throughout the world. Saccharomyces cerevisiae was used to alleviate porcine jejunal epithelia cell (IPEC-J2) injury induced by DON in this study. The results indicated that cell viability and proliferation rates were significantly decreased when DON concentrations were increased from 0 to 64 µM after 24 h incubation (p < 0.05). The longer incubation time and higher DON concentrations would cause more serious effects on cell viability. S. cerevisiae could significantly degrade DON and decrease lactic dehydrogenase (LDH) release in the cells induced by DON (p < 0.05). DON (4 µM) could increase necrotic and apoptotic cell rates as well as decrease viable cell rates, compared with the control group (p < 0.05). However, S. cerevisiae addition in the DON group could decrease necrotic, late apoptotic and early apoptotic cell rates by 38.05%, 46.37% and 44.78% respectively, increase viable cell rates by 2.35%, compared with the single DON group (p < 0.05). In addition, S. cerevisiae addition could up-regulate mRNA abundances of IL-6, IL-8 and IL-10 in IPEC-J2 cells (p < 0.05), but down-regulate mRNA abundances of tight junction proteins (TJP-1) and occludin by 36.13% and 50.18% at 1 µM of DON (p < 0.05). It could be concluded that S. cerevisiae was able to alleviate IPEC-J2 cell damage exposed to DON.
脱氧雪腐镰刀菌烯醇（DON）是全球谷物类食品中最常见的霉菌毒素之一。本研究采用酿酒酵母来减轻DON诱导的猪空肠上皮细胞（IPEC-J2）损伤。结果表明，在24小时孵育后，当DON浓度从0增加到64µM时，细胞活力和增殖率显著降低（p<0.05）。孵育时间越长、DON浓度越高，对细胞活力的影响越严重。酿酒酵母能够显著降解DON，并降低DON诱导的细胞中乳酸脱氢酶（LDH）的释放（p<0.05）。与对照组相比，4µM的DON可增加坏死和凋亡细胞率，并降低活细胞率（p<0.05）。然而，与单一DON组相比，在DON组中添加酿酒酵母可使坏死、晚期凋亡和早期凋亡细胞率分别降低38.05%、46.37%和44.78%，活细胞率提高2.35%（p<0.05）。此外，添加酿酒酵母可上调IPEC-J2细胞中IL-6、IL-8和IL-10的mRNA丰度（p<0.05），但在1µM的DON作用下，紧密连接蛋白（TJP-1）和闭合蛋白的mRNA丰度分别下调36.13%和50.18%（p<0.05）。可以得出结论，酿酒酵母能够减轻暴露于DON的IPEC-J2细胞损伤。
