França Maria Elisa Duarte, Sinhorim Larissa, Martins Daniel Fernandes, Schleip Robert, Machado-Pereira Nicolas A M M, de Souza Gabriel Melo, Horewicz Verônica Vargas, Santos Gilmar Moraes
Posture and Balance Laboratory (LAPEQ), College of Health Sciences and Sports, Santa Catarina State University (UDESC), Florianópolis, Brazil.
Neurosciences Experimental Laboratory (LANEX), Postgraduate Program in Health Sciences (PPGCS), University of Southern Santa Catarina, Palhoça, Brazil.
Front Physiol. 2020 Dec 3;11:587373. doi: 10.3389/fphys.2020.587373. eCollection 2020.
Fascia can become rigid and assume a fibrotic pattern due to inflammatory processes. Manipulation of the fascial system (MFS), manual technique targeting connective tissues, is commonly used in clinical practice in pain management. We aimed to verify MFS effects on the connective tissue inflammatory changes in mice. Swiss male mice ( = 44) were distributed into groups: carrageenan without treatment (Car, = 11), carrageenan with MFS (Car + MFS, = 12), saline without treatment ( = 10), and saline with MFS (saline + MFS, = 11). Interleukin 4 (IL-4), IL-6, tumor necrosis factor (TNF), transforming growth factor β1 (TGF-β1), and monocyte chemoattractant protein 1 (MCP-1) levels were verified by enzyme-linked immunosorbent assay. Neutrophil (Ly-6G), macrophage (F4/80), and nitric oxide synthase 2 (NOS-2) were identified using Western blot. The MFS protocol was applied from the first to the third day after inflammation of the connective tissue of the thoracolumbar region. There was a significant MFS effect on IL-4 ( = 0.02) and TGF-β1 ( = 0.04), without increasing MCP-1, TNF, and IL-6 levels ( > 0.05) on thoracolumbar region from Car + MFS, in comparison with saline. Ly-6G in Car + MFS presented lower levels when compared with saline ( = 0.003) or saline + MFS (0.003). NOS-2 levels were lower in Car + MFS than in saline + MFS ( = 0.0195) or saline ( = 0.003). MFS may have an anti-inflammatory effect, based on TGF-β1 and IL-4. IL-4 may have inhibited neutrophil migration. Lower levels of NOS-2 may be linked to the lack of macrophages, which are responsible for NOS-2 expression.
由于炎症过程，筋膜会变得僵硬并呈现纤维化模式。筋膜系统手法治疗（MFS）是一种针对结缔组织的手法技术，在疼痛管理的临床实践中常用。我们旨在验证MFS对小鼠结缔组织炎症变化的影响。将瑞士雄性小鼠（n = 44）分为几组：未治疗的角叉菜胶组（Car，n = 11）、接受MFS的角叉菜胶组（Car + MFS，n = 12）、未治疗的生理盐水组（n = 10）和接受MFS的生理盐水组（生理盐水 + MFS，n = 11）。通过酶联免疫吸附测定法验证白细胞介素4（IL - 4）、IL - 6、肿瘤坏死因子（TNF）、转化生长因子β1（TGF - β1）和单核细胞趋化蛋白1（MCP - 1）水平。使用蛋白质免疫印迹法鉴定中性粒细胞（Ly - 6G）、巨噬细胞（F4/80）和一氧化氮合酶2（NOS - 2）。在胸腰段结缔组织炎症后的第一天至第三天应用MFS方案。与生理盐水相比，MFS对Car + MFS组胸腰段的IL - 4（P = 0.02）和TGF - β1（P = 0.04）有显著影响，而MCP - 1、TNF和IL - 6水平没有升高（P > 0.05）。与生理盐水（P = 0.003）或生理盐水 + MFS（P = 0.003）相比，Car + MFS组的Ly - 6G水平较低。Car + MFS组的NOS - 2水平低于生理盐水 + MFS组（P = 0.0195）或生理盐水组（P = 0.003）。基于TGF - β1和IL - 4，MFS可能具有抗炎作用。IL - 4可能抑制了中性粒细胞的迁移。较低的NOS - 2水平可能与缺乏负责NOS - 2表达的巨噬细胞有关。
