Mahmoud Usama T, Darwish Madeha H A, Ali Fatma Abo Zakaib, Amen Omar A, Mahmoud Manal A M, Ahmed Omar B, Abd El-Reda Ghada, Osman Mohamed A, Othman Aly A, Abushahba Mostafa F N, El Shoukary Ramadan D
Department of Animal and Poultry Behaviour and Management, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt.
Avian Pathol. 2021 Mar 29:1-13. doi: 10.1080/03079457.2021.1875123.
The current experiment was designed to evaluate the effects of dietary supplementations of zinc oxide nanoparticles (ZONPs) on some behavioural, performance, welfare and histopathological changes in broilers exposed to multidrug resistant (MRSA)-induced footpad dermatitis (FPD). Eighty-four male Indian River (IR) broilers were randomly allotted to six different dietary treatments as follows: C-ve, C+ve, 10, 20, 30 and 40 ppm ZONPs from 7 to 49d of age. At day 28, broilers ( = 70) were sub-cutaneously injected with 0.5 ml of saline containing 5.3 × 10CFU/ml of (MRSA) in each metatarsal foot pad. Control (non-infected) broilers were given 0.5 ml of saline ( = 14). Results clarified that non-infected birds and ZONPs-fed birds had significantly higher standing and feeding activities and lower resting activities in comparison with the infected group. Also, the infected group had significantly lower body weight gain (BWG) and higher feed conversion ratio (FCR) than the non-infected group. In addition, the non-infected birds and ZONPs groups had significantly lower object crossing and tonic immobility times (TI) and gait scores (GS) in comparison with the group. Only ZONPs 30, 40 ppm and non-infected groups had a significantly higher latency to lie time (LLT) and lower serum cortisol level in comparison with the group. Moreover, there were significant changes in the gross lesion score and histopathological lesions between the different groups. In conclusion, the dietary supplementation of ZONPs can reduce -induced negative effects of FPD in broilers.
当前实验旨在评估日粮添加氧化锌纳米颗粒（ZONPs）对感染耐多药金黄色葡萄球菌（MRSA）引起的脚垫皮炎（FPD）的肉鸡某些行为、生产性能、福利和组织病理学变化的影响。84只雄性印度河（IR）肉鸡从7日龄至49日龄被随机分配到六种不同的日粮处理组：阴性对照组、阳性对照组、10、20、30和40 ppm的ZONPs组。在第28天，70只肉鸡在每个跖部脚垫皮下注射0.5 ml含5.3×10CFU/ml MRSA的生理盐水。对照组（未感染）肉鸡注射0.5 ml生理盐水（n = 14）。结果表明，与感染组相比，未感染的鸡和饲喂ZONPs的鸡站立和采食活动显著增加，休息活动减少。此外，感染组的体重增加（BWG）显著低于未感染组，饲料转化率（FCR）显著高于未感染组。另外，与感染组相比，未感染的鸡和ZONPs组的穿越物体次数和强直性静止时间（TI）以及步态评分（GS）显著降低。只有30、40 ppm的ZONPs组和未感染组与感染组相比，躺卧潜伏期（LLT）显著延长，血清皮质醇水平显著降低。此外，不同组之间的大体病变评分和组织病理学病变存在显著差异。总之，日粮添加ZONPs可减轻MRSA诱导的肉鸡FPD的负面影响。
